Neurogenic differentiation of murine and human adipose-derived stromal cells

Kristine M. Safford, Kevin C. Hicok, Shawn D. Safford, Yuan Di C. Halvorsen, William O. Wilkison, Jeffrey M. Gimble, Henry E. Rice

Research output: Contribution to journalArticlepeer-review

671 Scopus citations

Abstract

The identification of cells capable of neuronal differentiation has great potential for cellular therapies. We examined whether murine and human adipose-derived adult stem (ADAS) cells can be induced to undergo neuronal differentiation. We isolated ADAS cells from the adipose tissue of adult BalbC mice or from human liposuction tissue and induced neuronal differentiation with valproic acid butylated hydroxyanisole insulin and hydrocortisone. As early as 1-3 h after neuronal induction the phenotype of ADAS cells changed towards neuronal morphology. Following neuronal induction muADAS cells displayed immunocytochemical staining for GFAP nestin and NeuN and huADAS cells displayed staining for intermediate filament M nestin and NeuN. Following neuronal induction of murine and human ADAS cells Western blot analysis confirmed GFAP nestin and NeuN protein expression. Pretreatment with EGF and basic FGF augmented the neuronal differentiation of huADAS cells. The neuronal differentiation of stromal cells from adipose tissue has broad biological and clinical implications.

Original languageEnglish (US)
Pages (from-to)371-379
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume294
Issue number2
DOIs
StatePublished - 2002

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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