Neuronal calcium sensor-1 gene ncs-1a is essential for semicircular canal formation in zebrafish inner ear

Brian Blasiole, Nadine Kabbani, Wendy Boehmler, Bernard Thisse, Christine Thisse, Victor Canfield, Robert Levenson

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

We have analyzed the functional role of neuronal calcium sensor-1 (Ncs-1) in zebrafish development. We identified two orthologs of the mammalian NCS-1 gene. Full-length cDNAs encoding zebrafish Ncs-1a and Ncs-1b polypeptides were cloned and characterized. Whole-mount in situ hybridization revealed that ncs-1a mRNA was expressed beginning at early somitogenesis. As development progressed, ncs-1a mRNA was present throughout the embryo with expression detected in ventral hematopoietic mesoderm, pronephric tubules, CNS nuclei, and otic vesicle. By 4.5 days post fertilization (dpf), ncs-1a expression was detected primarily in the brain. Expression of ncs-1b mRNA was first detected at 36 hours post fertilization (hpf) and was restricted to the olfactory bulb. By 4.5 dpf, ncs-1b was expressed at low levels throughout the brain. Knockdown of ncs-1a mRNA translation with antisense morpholinos blocked formation of semicircular canals. These studies identify a novel function for ncs-1a in inner ear development and suggest that this calcium sensor plays an important role in vestibular function.

Original languageEnglish (US)
Pages (from-to)285-297
Number of pages13
JournalJournal of Neurobiology
Volume64
Issue number3
DOIs
StatePublished - Sep 1 2005

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

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