Neuronal differentiation of retinoblastoma cells induced by medium conditioned by human RPE cells

J. Tombran-Tink, L. V. Johnson

Research output: Contribution to journalArticlepeer-review

252 Scopus citations

Abstract

Tumor cells can be induced to differentiate in vitro by biochemical manipulation of their culture environment. In the studies described here, the effects of medium conditioned by human retinal pigmented epithelial (RPE) cells on Y79 human retinoblastoma cells have been examined. RPE-conditioned medium in conjunction with laminin and a poly-D-lysine substratum is observed to induce neuronal differentiation of Y79 cells. The cells extend long cellular processes and exhibit immunologically detectable neurotypic properties. In contrast, control Y79 cells not exposed to medium conditioned by RPE cells exhibit only infrequent neuronal phenotypes. This response of Y79 cells to RPE-conditioned medium indicated that factors secreted by RPE cells can act as inducers of neuronal differentiation in retinoblastoma cells and suggest that such factors may be of importance in the development and differentiation of the neural retina.

Original languageEnglish (US)
Pages (from-to)1700-1707
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume30
Issue number8
StatePublished - 1989

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Neuronal differentiation of retinoblastoma cells induced by medium conditioned by human RPE cells'. Together they form a unique fingerprint.

Cite this