Neuronal differentiation of retinoblastoma cells induced by medium conditioned by human RPE cells

Joyce Tombran-Tink, L. V. Johnson

Research output: Contribution to journalArticle

246 Citations (Scopus)

Abstract

Tumor cells can be induced to differentiate in vitro by biochemical manipulation of their culture environment. In the studies described here, the effects of medium conditioned by human retinal pigmented epithelial (RPE) cells on Y79 human retinoblastoma cells have been examined. RPE-conditioned medium in conjunction with laminin and a poly-D-lysine substratum is observed to induce neuronal differentiation of Y79 cells. The cells extend long cellular processes and exhibit immunologically detectable neurotypic properties. In contrast, control Y79 cells not exposed to medium conditioned by RPE cells exhibit only infrequent neuronal phenotypes. This response of Y79 cells to RPE-conditioned medium indicated that factors secreted by RPE cells can act as inducers of neuronal differentiation in retinoblastoma cells and suggest that such factors may be of importance in the development and differentiation of the neural retina.

Original languageEnglish (US)
Pages (from-to)1700-1707
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume30
Issue number8
StatePublished - 1989

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Retinoblastoma
Conditioned Culture Medium
Cell Differentiation
Epithelial Cells
Laminin
Lysine
Retina
Phenotype
Neoplasms

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

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Neuronal differentiation of retinoblastoma cells induced by medium conditioned by human RPE cells. / Tombran-Tink, Joyce; Johnson, L. V.

In: Investigative Ophthalmology and Visual Science, Vol. 30, No. 8, 1989, p. 1700-1707.

Research output: Contribution to journalArticle

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