Neuronal subsets express multiple high-molecular-weight cell-surface glycoconjugates defined by monoclonal antibodies cat-301 and VC1.1

Sam Zaremba, Janice R. Naegele, Colin Barnstable, Susan Hockfield

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Cat-301 and VC1.1 are monoclonal antibodies that recognize surface-associated molecules on subsets of mammalian CNS neurons. Earlier work demonstrated that Cat-301 recognizes a 680-kDa chondroitin sulfate proteoglycan (PG). VC1.1 has been shown to recognize 3 polypeptide bands on Western blot analysis; a major band at 95-105 kDa and additional bands at 145 kDa and 170 kDa. In the present report, we show that VC1.1 also reacts with a high-molecular-weight glycoconjugate. Immunoprecipitation experiments and biochemical characterizations indicate that Cat-301 and VC1.1 define at least 3 distinct high-molecular-weight antigens. The VC1.1 antigens react with antikeratan sulfate antibodies, while the Cat-301 antigens do not. By immunodepletion, we show that some VC1.1 antigens are Cat-301 positive, while others are Cat-301 negative. In addition, Cat-301-reactive proteoglycans are heterogeneous with respect to the presence or absence of VC1.1 epitopes. Double-label immunofluorescence studies with these 2 antibodies are consistent with the biochemical results and show that there are 3 classes of immunoreactive neurons in the cat CNS: Cat-301+/VC1.1+, Cat-301-/VC1.1+, acid Cat-301+/VC1.1-, These results indicate that structural microheterogeneity exists among Cat-301 and VC1.1 high-molecular-weight glycoconjugates. This heterogeneity may be a reflection of the diverse neuronal phenotypes that are recognized by Cat-301 and VC1.1 in the mammalian CNS.

Original languageEnglish (US)
Pages (from-to)2985-2995
Number of pages11
JournalJournal of Neuroscience
Volume10
Issue number9
StatePublished - Dec 1 1990

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Glycoconjugates
Cats
Molecular Weight
Monoclonal Antibodies
Antigens
Chondroitin Sulfate Proteoglycans
Neurons
Antibodies
Proteoglycans
Immunoprecipitation
Sulfates
Fluorescent Antibody Technique
Epitopes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

@article{e4a5bd897df74c75ac83d387a69c61a5,
title = "Neuronal subsets express multiple high-molecular-weight cell-surface glycoconjugates defined by monoclonal antibodies cat-301 and VC1.1",
abstract = "Cat-301 and VC1.1 are monoclonal antibodies that recognize surface-associated molecules on subsets of mammalian CNS neurons. Earlier work demonstrated that Cat-301 recognizes a 680-kDa chondroitin sulfate proteoglycan (PG). VC1.1 has been shown to recognize 3 polypeptide bands on Western blot analysis; a major band at 95-105 kDa and additional bands at 145 kDa and 170 kDa. In the present report, we show that VC1.1 also reacts with a high-molecular-weight glycoconjugate. Immunoprecipitation experiments and biochemical characterizations indicate that Cat-301 and VC1.1 define at least 3 distinct high-molecular-weight antigens. The VC1.1 antigens react with antikeratan sulfate antibodies, while the Cat-301 antigens do not. By immunodepletion, we show that some VC1.1 antigens are Cat-301 positive, while others are Cat-301 negative. In addition, Cat-301-reactive proteoglycans are heterogeneous with respect to the presence or absence of VC1.1 epitopes. Double-label immunofluorescence studies with these 2 antibodies are consistent with the biochemical results and show that there are 3 classes of immunoreactive neurons in the cat CNS: Cat-301+/VC1.1+, Cat-301-/VC1.1+, acid Cat-301+/VC1.1-, These results indicate that structural microheterogeneity exists among Cat-301 and VC1.1 high-molecular-weight glycoconjugates. This heterogeneity may be a reflection of the diverse neuronal phenotypes that are recognized by Cat-301 and VC1.1 in the mammalian CNS.",
author = "Sam Zaremba and Naegele, {Janice R.} and Colin Barnstable and Susan Hockfield",
year = "1990",
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Neuronal subsets express multiple high-molecular-weight cell-surface glycoconjugates defined by monoclonal antibodies cat-301 and VC1.1. / Zaremba, Sam; Naegele, Janice R.; Barnstable, Colin; Hockfield, Susan.

In: Journal of Neuroscience, Vol. 10, No. 9, 01.12.1990, p. 2985-2995.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Neuronal subsets express multiple high-molecular-weight cell-surface glycoconjugates defined by monoclonal antibodies cat-301 and VC1.1

AU - Zaremba, Sam

AU - Naegele, Janice R.

AU - Barnstable, Colin

AU - Hockfield, Susan

PY - 1990/12/1

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N2 - Cat-301 and VC1.1 are monoclonal antibodies that recognize surface-associated molecules on subsets of mammalian CNS neurons. Earlier work demonstrated that Cat-301 recognizes a 680-kDa chondroitin sulfate proteoglycan (PG). VC1.1 has been shown to recognize 3 polypeptide bands on Western blot analysis; a major band at 95-105 kDa and additional bands at 145 kDa and 170 kDa. In the present report, we show that VC1.1 also reacts with a high-molecular-weight glycoconjugate. Immunoprecipitation experiments and biochemical characterizations indicate that Cat-301 and VC1.1 define at least 3 distinct high-molecular-weight antigens. The VC1.1 antigens react with antikeratan sulfate antibodies, while the Cat-301 antigens do not. By immunodepletion, we show that some VC1.1 antigens are Cat-301 positive, while others are Cat-301 negative. In addition, Cat-301-reactive proteoglycans are heterogeneous with respect to the presence or absence of VC1.1 epitopes. Double-label immunofluorescence studies with these 2 antibodies are consistent with the biochemical results and show that there are 3 classes of immunoreactive neurons in the cat CNS: Cat-301+/VC1.1+, Cat-301-/VC1.1+, acid Cat-301+/VC1.1-, These results indicate that structural microheterogeneity exists among Cat-301 and VC1.1 high-molecular-weight glycoconjugates. This heterogeneity may be a reflection of the diverse neuronal phenotypes that are recognized by Cat-301 and VC1.1 in the mammalian CNS.

AB - Cat-301 and VC1.1 are monoclonal antibodies that recognize surface-associated molecules on subsets of mammalian CNS neurons. Earlier work demonstrated that Cat-301 recognizes a 680-kDa chondroitin sulfate proteoglycan (PG). VC1.1 has been shown to recognize 3 polypeptide bands on Western blot analysis; a major band at 95-105 kDa and additional bands at 145 kDa and 170 kDa. In the present report, we show that VC1.1 also reacts with a high-molecular-weight glycoconjugate. Immunoprecipitation experiments and biochemical characterizations indicate that Cat-301 and VC1.1 define at least 3 distinct high-molecular-weight antigens. The VC1.1 antigens react with antikeratan sulfate antibodies, while the Cat-301 antigens do not. By immunodepletion, we show that some VC1.1 antigens are Cat-301 positive, while others are Cat-301 negative. In addition, Cat-301-reactive proteoglycans are heterogeneous with respect to the presence or absence of VC1.1 epitopes. Double-label immunofluorescence studies with these 2 antibodies are consistent with the biochemical results and show that there are 3 classes of immunoreactive neurons in the cat CNS: Cat-301+/VC1.1+, Cat-301-/VC1.1+, acid Cat-301+/VC1.1-, These results indicate that structural microheterogeneity exists among Cat-301 and VC1.1 high-molecular-weight glycoconjugates. This heterogeneity may be a reflection of the diverse neuronal phenotypes that are recognized by Cat-301 and VC1.1 in the mammalian CNS.

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