Neuron‐specific enolase levels in primary cultures of neurons

C. ‐L Schengrund, P. J. Marangos

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Primary neuronal cell cultures, derived by centrifugal elutriation of cells dissociated from embryonic rat cerebra, have been analyzed for their content of neuron‐specific enolase (NSE) and non‐neuronal enolase (NNE). Both immunocytochemical staining and radioimmunoassay establish that NSE is present in these cells. Furthermore, the observed increase in NSE and decrease in the NNE/NSE ratio with time in culture parallels that observed in vivo. It has recently been shown that during in vivo neurogenesis and maturation a “switch over” occurs from NNE to NSE which correlates with neuronal differentiation. The presence of a neuron‐specific protein in these primary neuronal cultures and the changes observed during growth further support their use as a model system for the study of nervous tissue differentiation.

Original languageEnglish (US)
Pages (from-to)305-311
Number of pages7
JournalJournal of Neuroscience Research
Volume5
Issue number4
DOIs
StatePublished - 1980

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Phosphopyruvate Hydratase
Neurons
Nerve Tissue
Primary Cell Culture
Neurogenesis
Cerebrum
Radioimmunoassay
Staining and Labeling
Growth

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

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abstract = "Primary neuronal cell cultures, derived by centrifugal elutriation of cells dissociated from embryonic rat cerebra, have been analyzed for their content of neuron‐specific enolase (NSE) and non‐neuronal enolase (NNE). Both immunocytochemical staining and radioimmunoassay establish that NSE is present in these cells. Furthermore, the observed increase in NSE and decrease in the NNE/NSE ratio with time in culture parallels that observed in vivo. It has recently been shown that during in vivo neurogenesis and maturation a “switch over” occurs from NNE to NSE which correlates with neuronal differentiation. The presence of a neuron‐specific protein in these primary neuronal cultures and the changes observed during growth further support their use as a model system for the study of nervous tissue differentiation.",
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Neuron‐specific enolase levels in primary cultures of neurons. / Schengrund, C. ‐L; Marangos, P. J.

In: Journal of Neuroscience Research, Vol. 5, No. 4, 1980, p. 305-311.

Research output: Contribution to journalArticle

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AU - Schengrund, C. ‐L

AU - Marangos, P. J.

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