Neuropeptide Y hyperpolarizes submucosal neurons of the guinea-pig descending colon

Keiji Hirai, Kirsteen Browning, Gordon M. Lees

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Effects of neuropeptide Y (NPY) on submucosal neurons of the guinea-pig descending colon were investigated electrophysiologically by means of intracellular electrophysiological recordings. NPY (100 nM) induced a marked and prolonged hyperpolarization, accompanied by a decrease in input resistance in most (90%) neurons. This NPY-induced hyperpolarization was diminished and augmented by membrane hyperpolarization and depolarization, respectively. The NPY-hyperpolarization was not affected by exposure to either calcium-free solu solutions or the α2-adrenoceptor antagonist, idazoxan (1 μM). When more than one peptide was applied to a neuron, NPY, PYY and Pro34-NPY were equipotent, whilst NPY13-36 was less potent. It was concluded that NPY hyperpolarized submucosal neurons of the guinea-pig descending colon, possibly via a direct action on postsynaptic Y1-receptor and increasing potassium conductance.

Original languageEnglish (US)
Pages (from-to)212-214
Number of pages3
JournalNeuroscience letters
Volume227
Issue number3
DOIs
StatePublished - May 23 1997

Fingerprint

Descending Colon
Neuropeptide Y
Guinea Pigs
Neurons
Idazoxan
Adrenergic Receptors
Potassium
Calcium
Peptides
Membranes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

@article{ce8a121e8a5943c0853a2348f93c32f0,
title = "Neuropeptide Y hyperpolarizes submucosal neurons of the guinea-pig descending colon",
abstract = "Effects of neuropeptide Y (NPY) on submucosal neurons of the guinea-pig descending colon were investigated electrophysiologically by means of intracellular electrophysiological recordings. NPY (100 nM) induced a marked and prolonged hyperpolarization, accompanied by a decrease in input resistance in most (90{\%}) neurons. This NPY-induced hyperpolarization was diminished and augmented by membrane hyperpolarization and depolarization, respectively. The NPY-hyperpolarization was not affected by exposure to either calcium-free solu solutions or the α2-adrenoceptor antagonist, idazoxan (1 μM). When more than one peptide was applied to a neuron, NPY, PYY and Pro34-NPY were equipotent, whilst NPY13-36 was less potent. It was concluded that NPY hyperpolarized submucosal neurons of the guinea-pig descending colon, possibly via a direct action on postsynaptic Y1-receptor and increasing potassium conductance.",
author = "Keiji Hirai and Kirsteen Browning and Lees, {Gordon M.}",
year = "1997",
month = "5",
day = "23",
doi = "10.1016/S0304-3940(97)00325-X",
language = "English (US)",
volume = "227",
pages = "212--214",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

Neuropeptide Y hyperpolarizes submucosal neurons of the guinea-pig descending colon. / Hirai, Keiji; Browning, Kirsteen; Lees, Gordon M.

In: Neuroscience letters, Vol. 227, No. 3, 23.05.1997, p. 212-214.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Neuropeptide Y hyperpolarizes submucosal neurons of the guinea-pig descending colon

AU - Hirai, Keiji

AU - Browning, Kirsteen

AU - Lees, Gordon M.

PY - 1997/5/23

Y1 - 1997/5/23

N2 - Effects of neuropeptide Y (NPY) on submucosal neurons of the guinea-pig descending colon were investigated electrophysiologically by means of intracellular electrophysiological recordings. NPY (100 nM) induced a marked and prolonged hyperpolarization, accompanied by a decrease in input resistance in most (90%) neurons. This NPY-induced hyperpolarization was diminished and augmented by membrane hyperpolarization and depolarization, respectively. The NPY-hyperpolarization was not affected by exposure to either calcium-free solu solutions or the α2-adrenoceptor antagonist, idazoxan (1 μM). When more than one peptide was applied to a neuron, NPY, PYY and Pro34-NPY were equipotent, whilst NPY13-36 was less potent. It was concluded that NPY hyperpolarized submucosal neurons of the guinea-pig descending colon, possibly via a direct action on postsynaptic Y1-receptor and increasing potassium conductance.

AB - Effects of neuropeptide Y (NPY) on submucosal neurons of the guinea-pig descending colon were investigated electrophysiologically by means of intracellular electrophysiological recordings. NPY (100 nM) induced a marked and prolonged hyperpolarization, accompanied by a decrease in input resistance in most (90%) neurons. This NPY-induced hyperpolarization was diminished and augmented by membrane hyperpolarization and depolarization, respectively. The NPY-hyperpolarization was not affected by exposure to either calcium-free solu solutions or the α2-adrenoceptor antagonist, idazoxan (1 μM). When more than one peptide was applied to a neuron, NPY, PYY and Pro34-NPY were equipotent, whilst NPY13-36 was less potent. It was concluded that NPY hyperpolarized submucosal neurons of the guinea-pig descending colon, possibly via a direct action on postsynaptic Y1-receptor and increasing potassium conductance.

UR - http://www.scopus.com/inward/record.url?scp=0030907083&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030907083&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(97)00325-X

DO - 10.1016/S0304-3940(97)00325-X

M3 - Article

VL - 227

SP - 212

EP - 214

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -