Neutralization of IL-4 and IFN-γ facilitates inducing TGF-β-induced CD4+Foxp3+ regulatory cells

Xiaojuan Tao, Jilin Ma, Yonghua Zhang, Jianning Yu, Long Cai, Juhua Wang, Song Guo Zheng

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

It has been well recognized that TGF-β is able to induce CD4+CD25+Foxp3+ suppressor/regulatory T (iTreg) cells and IL-2 facilitates iTreg induction and expansion, however, only half of TGF-β-induced CD4+CD25+ cells express Foxp3 and remaining CD4+CD25+Foxp3- cells may represent effector cells. Whether other factor(s) can increase Foxp3 expression by CD4+CD25+ cells induced with TGF-β is still unclear. Here we show that addition of exogenous IFN-γ or IL-4 diminished the ability of TGF-β to induce Foxp3 expression and IL-2 failed to rescue this decreased Foxp3 expression. Conversely, neutralization of IFN-γ and IL-4 significantly enhanced the ability of TGF-β to induce Foxp3 and develop the suppressive activity, indicating that different cytokine profiles affect the differentiation of CD4+CD25Foxp3+ subset induced by TGF-β. These results show that combination of antibodies against IFN-γ and IL-4 and TGF-β enhances the efficacy of generation and function of iTreg cells and may therefore provide a novel therapeutic strategy for the treatment of autoimmune and other chronic inflammatory diseases.

Original languageEnglish (US)
Pages (from-to)52-57
Number of pages6
JournalInternational Journal of Biomedical Science
Volume4
Issue number1
StatePublished - Mar 1 2008

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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