Neutravidin micropatterning by deep UV irradiation

Ying Ming Huang, Maruti Uppalapati, William Olaf Hancock, Thomas Nelson Jackson

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

We describe a novel approach for directly patterning neutravidin protein by exposure to deep UV irradiation. Neutravidin is physically absorbed onto the glass or quartz substrate, dehydrated in acetone and air-dried. Dry neutravidin-coated samples are patterned either by top-side or back-side exposure to 185 nm UV. Subsequent introduction of fluorescent biotinylated proteins clearly demonstrates binding to the masked neutravidin regions and no binding to exposed areas. Patterned samples retain their protein affinity for at least three months of storage at room temperature.

Original languageEnglish (US)
Pages (from-to)1745-1747
Number of pages3
JournalLab on a Chip
Volume8
Issue number10
DOIs
StatePublished - 2008

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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