Neutron diffraction study of pseudomonas aeruginosa lipopolysaccharide bilayers

Thomas Abraham, Sarah R. Schooling, Mu Ping Nieh, Norbert Kučerka, Terry J. Beveridge, John Katsaras

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Lipopolysaccharides (LPSs) are a major class of macromolecules populating the surface of Gram-negative bacteria. They contribute significantly to the bacterium's surface properties and play a crucial role in regulating the permeability of its outer membrane. Here, we report on neutron diffraction studies performed on aligned, self-assembled bilayers of LPS isolated from Pseudomonas aeruginosa PAO1. This LPS system is comprised of a mixture of rough and smooth A-band and B-band LPS, similar to that naturally found in P. aeruginosa. Temperature scans were conducted at various levels of hydration, and the phases adopted by LPS, along with their corresponding transition temperatures, have been identified. Because of LPS's chemical heterogeneity, the gel-to-liquid-crystalline transition was continuous and not abrupt as commonly observed in single-component phospholipid systems. From the construction of one-dimensional scattering length density profiles, we find that water penetrates into the hydrocarbon region up to and including the center of liquid-crystalline LPS bilayers. This permeability to water also extends to bilayers in the continuous phase transition region and could have far-reaching implications as to how small molecules penetrate the outer membrane of Gram-negative bacteria.

Original languageEnglish (US)
Pages (from-to)2477-2483
Number of pages7
JournalJournal of Physical Chemistry B
Volume111
Issue number10
DOIs
StatePublished - Mar 15 2007

Fingerprint

pseudomonas
Neutron diffraction
bacteria
Lipopolysaccharides
neutron diffraction
Bacteria
permeability
Crystalline materials
membranes
Membranes
Phospholipids
Liquids
liquids
Macromolecules
macromolecules
Hydration
surface properties
water
Superconducting transition temperature
Surface properties

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Abraham, T., Schooling, S. R., Nieh, M. P., Kučerka, N., Beveridge, T. J., & Katsaras, J. (2007). Neutron diffraction study of pseudomonas aeruginosa lipopolysaccharide bilayers. Journal of Physical Chemistry B, 111(10), 2477-2483. https://doi.org/10.1021/jp066012
Abraham, Thomas ; Schooling, Sarah R. ; Nieh, Mu Ping ; Kučerka, Norbert ; Beveridge, Terry J. ; Katsaras, John. / Neutron diffraction study of pseudomonas aeruginosa lipopolysaccharide bilayers. In: Journal of Physical Chemistry B. 2007 ; Vol. 111, No. 10. pp. 2477-2483.
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Abraham, T, Schooling, SR, Nieh, MP, Kučerka, N, Beveridge, TJ & Katsaras, J 2007, 'Neutron diffraction study of pseudomonas aeruginosa lipopolysaccharide bilayers', Journal of Physical Chemistry B, vol. 111, no. 10, pp. 2477-2483. https://doi.org/10.1021/jp066012

Neutron diffraction study of pseudomonas aeruginosa lipopolysaccharide bilayers. / Abraham, Thomas; Schooling, Sarah R.; Nieh, Mu Ping; Kučerka, Norbert; Beveridge, Terry J.; Katsaras, John.

In: Journal of Physical Chemistry B, Vol. 111, No. 10, 15.03.2007, p. 2477-2483.

Research output: Contribution to journalArticle

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