Neutrophil proteinases in hydrochloric acid- and endotoxin-induced acute lung injury: Evaluation of interstitial protease activity by in situ zymography

Andry Van de Louw, Daniel Jean, Eric Frisdal, Charles Cerf, Marie Pia d’ortho, Andrew H. Baker, Chantal Lafuma, Philippe Duvaldestin, Alain Harf, Christophe Delclaux

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20 Scopus citations

Abstract

We investigate the role of polymorphonuclear neutrophil (PMN) proteinases, elastase, and gelatinase B in rat models of acute lung injury. Three groups of rats were studied after 6 hours after unilateral instillation of hydrochloric acid (HCl; 0.1 N), lipopolysaccharide (LPS) (4 μg), or saline. The results demonstrated that HCl-induced lung injury as compared with LPS-induced lung injury, was associated with an increase in permeability (wet/dry weight ratio and proteins in bronchoalveolar lavage fluid). In contrast, there was similar PMN recruitment (in bronchoalveolar lavage fluid and myeloperoxidase activity in lung homogenates) and similar proteinase exocytosis residual alveolar PMN content of elastase and gelatinase B) in both types of lung injury. In situ zymography, evaluating interstitial protease/inhibitor balance, demonstrated a decrease in gelatinolytic activity in both HCl and LPS-injured lungs compared with normal lung. The increase in interleukin 6 concentration in lung homogenates, which is observed after both injuries compared with saline-instilled animals, could be involved in up-regulation of tissue inhibitor of matrix metalloproteinase-1, shown by immunocytochemistry to participate in antiproteinase excess. Neither inhibition of alveolar neutrophil influx using leukocyte elastase inhibitor (EPI-hNE-4) nor inhibition of gelatinase activities by recombinant adenovirus for the human tissue inhibitor of matrix metalloproteinase-1 gene transfer decreased lung edema in HCl-induced injury. These data suggest the PMN proteinase do not contribute to HCl-induced acute lung injury in rats.

Original languageEnglish (US)
Pages (from-to)133-145
Number of pages13
JournalLaboratory Investigation
Volume82
Issue number2
DOIs
StatePublished - Feb 2002

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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