Neutrophils invade lumbar dorsal root ganglia after chronic constriction injury of the sciatic nerve

N. Morin, S. A. Owolabi, M. W. Harty, E. F. Papa, T. F. Tracy, S. K. Shaw, M. Kim, C. Y. Saab

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

To test whether neutrophils (PMN) target lumbar dorsal root ganglia (DRG) following axonal injury leading to neuropathic pain, we visualized PMN infiltration in DRG tissue sections and estimated PMN count by flow cytometry following sciatic chronic constriction injury (CCI). Seven days after CCI, results show PMN within DRG where their count increased by three fold ipsilateral to injury compared to contralateral or sham, concomitant with peak neuropathic pain behavior. Superoxide burst in PMN isolated from rats d7 after CCI was elevated by 170% ± 18 compared to naïve and MCP-1 mRNA expression in DRG increased by 8.9 ± 2.9 fold, but that of MIP-2, CINC-1, and RANTES did not change. We conclude that CCI causes PMN invasion of the DRG whereby the functional implication of their close proximity to neuronal axon and soma remains unknown.

Original languageEnglish (US)
Pages (from-to)164-171
Number of pages8
JournalJournal of Neuroimmunology
Volume184
Issue number1-2
DOIs
StatePublished - Mar 1 2007

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Spinal Ganglia
Sciatic Nerve
Constriction
Neutrophils
Wounds and Injuries
Neuralgia
Chemokine CCL5
Carisoprodol
Superoxides
Axons
Flow Cytometry
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

Morin, N. ; Owolabi, S. A. ; Harty, M. W. ; Papa, E. F. ; Tracy, T. F. ; Shaw, S. K. ; Kim, M. ; Saab, C. Y. / Neutrophils invade lumbar dorsal root ganglia after chronic constriction injury of the sciatic nerve. In: Journal of Neuroimmunology. 2007 ; Vol. 184, No. 1-2. pp. 164-171.
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Neutrophils invade lumbar dorsal root ganglia after chronic constriction injury of the sciatic nerve. / Morin, N.; Owolabi, S. A.; Harty, M. W.; Papa, E. F.; Tracy, T. F.; Shaw, S. K.; Kim, M.; Saab, C. Y.

In: Journal of Neuroimmunology, Vol. 184, No. 1-2, 01.03.2007, p. 164-171.

Research output: Contribution to journalArticle

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