New assay technologies for high-throughput screening

Lauren Silverman, Robert Campbell, James R. Broach

Research output: Contribution to journalArticlepeer-review

132 Scopus citations

Abstract

The use of high-throughput screening for early stage drug discovery imposes several constraints on the format of assays for therapeutic targets of interest. Homogeneous cell-free assays based on energy transfer, fluorescence polarization spectroscopy or fluorescence correlation spectroscopy provide the sensitivity, ease, speed and resistance to interference from test compounds needed to function in a high-throughput screening mode. Similarly, novel cell-based assays are now being adapted for high-throughput screening, providing for in situ analysis of a variety of biological targets. Finally, recent advances in assay miniaturization mark a transition to ultra high-throughput screening, ensuring that identification of lead compounds will not be the rate-limiting step in finding new drugs.

Original languageEnglish (US)
Pages (from-to)397-403
Number of pages7
JournalCurrent Opinion in Chemical Biology
Volume2
Issue number3
DOIs
StatePublished - Jun 1998

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

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