New assay technologies for high-throughput screening

Lauren Silverman, Robert Campbell, James Broach

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

The use of high-throughput screening for early stage drug discovery imposes several constraints on the format of assays for therapeutic targets of interest. Homogeneous cell-free assays based on energy transfer, fluorescence polarization spectroscopy or fluorescence correlation spectroscopy provide the sensitivity, ease, speed and resistance to interference from test compounds needed to function in a high-throughput screening mode. Similarly, novel cell-based assays are now being adapted for high-throughput screening, providing for in situ analysis of a variety of biological targets. Finally, recent advances in assay miniaturization mark a transition to ultra high-throughput screening, ensuring that identification of lead compounds will not be the rate-limiting step in finding new drugs.

Original languageEnglish (US)
Pages (from-to)397-403
Number of pages7
JournalCurrent Opinion in Chemical Biology
Volume2
Issue number3
DOIs
StatePublished - Jan 1 1998

Fingerprint

High-Throughput Screening Assays
Fluorescence Spectrometry
Assays
Screening
Throughput
Technology
Miniaturization
Fluorescence Polarization
Energy Transfer
Drug Discovery
Fluorescence
Spectroscopy
Lead compounds
Pharmaceutical Preparations
Energy transfer
Polarization
Therapeutics
Lead

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

Silverman, Lauren ; Campbell, Robert ; Broach, James. / New assay technologies for high-throughput screening. In: Current Opinion in Chemical Biology. 1998 ; Vol. 2, No. 3. pp. 397-403.
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New assay technologies for high-throughput screening. / Silverman, Lauren; Campbell, Robert; Broach, James.

In: Current Opinion in Chemical Biology, Vol. 2, No. 3, 01.01.1998, p. 397-403.

Research output: Contribution to journalArticle

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