New conditions for generation of gynogenetic half-tetrad embryos in the zebrafish (Danio rerio)

E. E. Gestl, E. J. Kauffman, J. L. Moore, Keith Cheng

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The generation of gynogenetic half-tetrads is an important tool for genetic mapping and mutant screens in zebrafish (Danio rerio). Half-tetrad gynogenesis can be accomplished using hydraulic pressure to disrupt microtubule-mediated segregation during meiosis II, which normally occurs after fertilization. However, the equipment used in the original studies is no longer available, and the reported pressures have not proven effective. Here, microsatellite markers were employed in a redetermination of the pressures required to generate zebrafish half-tetrads with currently available equipment. Two criteria were used to choose conditions for half tetrad gynogenesis: expected ploidy as confirmed with microsatellite markers and maximal egg viability. Pressures between 6140 and 6840 psi generate half- tetrad embryos, with optimal viabilities attained at 6140 and 6240 psi. As predicted, early pressure treatment of eggs fertilized with nonirradiated sperm created triploid embryos.

Original languageEnglish (US)
Pages (from-to)76-79
Number of pages4
JournalJournal of Heredity
Volume88
Issue number1
DOIs
StatePublished - Jan 1 1997

Fingerprint

Zebrafish
Embryonic Structures
Pressure
Microsatellite Repeats
Triploidy
Equipment and Supplies
Zygote
Ploidies
Meiosis
Fertilization
Microtubules
Ovum
Spermatozoa

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Gestl, E. E. ; Kauffman, E. J. ; Moore, J. L. ; Cheng, Keith. / New conditions for generation of gynogenetic half-tetrad embryos in the zebrafish (Danio rerio). In: Journal of Heredity. 1997 ; Vol. 88, No. 1. pp. 76-79.
@article{255403b59dce47a2b96caf72a9594076,
title = "New conditions for generation of gynogenetic half-tetrad embryos in the zebrafish (Danio rerio)",
abstract = "The generation of gynogenetic half-tetrads is an important tool for genetic mapping and mutant screens in zebrafish (Danio rerio). Half-tetrad gynogenesis can be accomplished using hydraulic pressure to disrupt microtubule-mediated segregation during meiosis II, which normally occurs after fertilization. However, the equipment used in the original studies is no longer available, and the reported pressures have not proven effective. Here, microsatellite markers were employed in a redetermination of the pressures required to generate zebrafish half-tetrads with currently available equipment. Two criteria were used to choose conditions for half tetrad gynogenesis: expected ploidy as confirmed with microsatellite markers and maximal egg viability. Pressures between 6140 and 6840 psi generate half- tetrad embryos, with optimal viabilities attained at 6140 and 6240 psi. As predicted, early pressure treatment of eggs fertilized with nonirradiated sperm created triploid embryos.",
author = "Gestl, {E. E.} and Kauffman, {E. J.} and Moore, {J. L.} and Keith Cheng",
year = "1997",
month = "1",
day = "1",
doi = "10.1093/oxfordjournals.jhered.a023063",
language = "English (US)",
volume = "88",
pages = "76--79",
journal = "Journal of Heredity",
issn = "0022-1503",
publisher = "Oxford University Press",
number = "1",

}

New conditions for generation of gynogenetic half-tetrad embryos in the zebrafish (Danio rerio). / Gestl, E. E.; Kauffman, E. J.; Moore, J. L.; Cheng, Keith.

In: Journal of Heredity, Vol. 88, No. 1, 01.01.1997, p. 76-79.

Research output: Contribution to journalArticle

TY - JOUR

T1 - New conditions for generation of gynogenetic half-tetrad embryos in the zebrafish (Danio rerio)

AU - Gestl, E. E.

AU - Kauffman, E. J.

AU - Moore, J. L.

AU - Cheng, Keith

PY - 1997/1/1

Y1 - 1997/1/1

N2 - The generation of gynogenetic half-tetrads is an important tool for genetic mapping and mutant screens in zebrafish (Danio rerio). Half-tetrad gynogenesis can be accomplished using hydraulic pressure to disrupt microtubule-mediated segregation during meiosis II, which normally occurs after fertilization. However, the equipment used in the original studies is no longer available, and the reported pressures have not proven effective. Here, microsatellite markers were employed in a redetermination of the pressures required to generate zebrafish half-tetrads with currently available equipment. Two criteria were used to choose conditions for half tetrad gynogenesis: expected ploidy as confirmed with microsatellite markers and maximal egg viability. Pressures between 6140 and 6840 psi generate half- tetrad embryos, with optimal viabilities attained at 6140 and 6240 psi. As predicted, early pressure treatment of eggs fertilized with nonirradiated sperm created triploid embryos.

AB - The generation of gynogenetic half-tetrads is an important tool for genetic mapping and mutant screens in zebrafish (Danio rerio). Half-tetrad gynogenesis can be accomplished using hydraulic pressure to disrupt microtubule-mediated segregation during meiosis II, which normally occurs after fertilization. However, the equipment used in the original studies is no longer available, and the reported pressures have not proven effective. Here, microsatellite markers were employed in a redetermination of the pressures required to generate zebrafish half-tetrads with currently available equipment. Two criteria were used to choose conditions for half tetrad gynogenesis: expected ploidy as confirmed with microsatellite markers and maximal egg viability. Pressures between 6140 and 6840 psi generate half- tetrad embryos, with optimal viabilities attained at 6140 and 6240 psi. As predicted, early pressure treatment of eggs fertilized with nonirradiated sperm created triploid embryos.

UR - http://www.scopus.com/inward/record.url?scp=0031059883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031059883&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.jhered.a023063

DO - 10.1093/oxfordjournals.jhered.a023063

M3 - Article

AN - SCOPUS:0031059883

VL - 88

SP - 76

EP - 79

JO - Journal of Heredity

JF - Journal of Heredity

SN - 0022-1503

IS - 1

ER -