New rapid method to detect BCR-ABL fusion genes with multiplex RT-qPCR in one-tube at a time

Yong Qing Tong, Zhi Jun Zhao, Bei Liu, An Yu Bao, Hong Yun Zheng, Jian Gu, Ying Xia, Mary McGrath, Sinisa Dovat, Chunhua Song, Yan Li

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fast identification of BCR-ABL fusion genes is critical for precise diagnosis, risk stratification and therapy scheme selection in leukemia. More convenient methods are needed for quickly detection of the BCR-ABL fusion genes. Multiplex fluorescent reverse transcription quantitative real-time PCR (Multiplex RT-qPCR) methods are developed for detection of the at least 14 subtypes of BCR-ABL fusion genes in one tube at a time by using patients’ bone marrow samples. The new Multiplex RT-qPCR method could quickly detect BCR-ABL fusion genes with sensitivity up to 10–10 6 copies. It can detect the fusion genes in patients’ bone marrow samples containing any subtypes of the major bcr (M-bcr) e13a2, e14a2, e13a3 and e14a3, the minor bcr (m-bcr) e1a2 and e1a3, the micro bcr (μ-bcr) e19a2 and e19a3, and the nano bcr (n-bcr) e6a2 and e6a3. The specificity is comparable to the FISH methods. The cutoff for clinical diagnosis of BCR-ABL(+) is also determined by testing in clinical chronic myeloid leukemia samples. This is a new fast method with high sensitivity and specificity for clinical detection of BCR-ABL fusion genes. It will benefit the precise diagnosis, targeted therapy and minimal residual disease (MRD) monitoring in leukemia.

Original languageEnglish (US)
Pages (from-to)47-53
Number of pages7
JournalLeukemia Research
Volume69
DOIs
StatePublished - Jun 1 2018

Fingerprint

Gene Fusion
Reverse Transcription
Real-Time Polymerase Chain Reaction
Leukemia
Bone Marrow
Residual Neoplasm
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Sensitivity and Specificity
Therapeutics

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Tong, Y. Q., Zhao, Z. J., Liu, B., Bao, A. Y., Zheng, H. Y., Gu, J., ... Li, Y. (2018). New rapid method to detect BCR-ABL fusion genes with multiplex RT-qPCR in one-tube at a time. Leukemia Research, 69, 47-53. https://doi.org/10.1016/j.leukres.2018.04.001
Tong, Yong Qing ; Zhao, Zhi Jun ; Liu, Bei ; Bao, An Yu ; Zheng, Hong Yun ; Gu, Jian ; Xia, Ying ; McGrath, Mary ; Dovat, Sinisa ; Song, Chunhua ; Li, Yan. / New rapid method to detect BCR-ABL fusion genes with multiplex RT-qPCR in one-tube at a time. In: Leukemia Research. 2018 ; Vol. 69. pp. 47-53.
@article{f184ba306c9a4e5487d81f8456fe8ee0,
title = "New rapid method to detect BCR-ABL fusion genes with multiplex RT-qPCR in one-tube at a time",
abstract = "Fast identification of BCR-ABL fusion genes is critical for precise diagnosis, risk stratification and therapy scheme selection in leukemia. More convenient methods are needed for quickly detection of the BCR-ABL fusion genes. Multiplex fluorescent reverse transcription quantitative real-time PCR (Multiplex RT-qPCR) methods are developed for detection of the at least 14 subtypes of BCR-ABL fusion genes in one tube at a time by using patients’ bone marrow samples. The new Multiplex RT-qPCR method could quickly detect BCR-ABL fusion genes with sensitivity up to 10–10 6 copies. It can detect the fusion genes in patients’ bone marrow samples containing any subtypes of the major bcr (M-bcr) e13a2, e14a2, e13a3 and e14a3, the minor bcr (m-bcr) e1a2 and e1a3, the micro bcr (μ-bcr) e19a2 and e19a3, and the nano bcr (n-bcr) e6a2 and e6a3. The specificity is comparable to the FISH methods. The cutoff for clinical diagnosis of BCR-ABL(+) is also determined by testing in clinical chronic myeloid leukemia samples. This is a new fast method with high sensitivity and specificity for clinical detection of BCR-ABL fusion genes. It will benefit the precise diagnosis, targeted therapy and minimal residual disease (MRD) monitoring in leukemia.",
author = "Tong, {Yong Qing} and Zhao, {Zhi Jun} and Bei Liu and Bao, {An Yu} and Zheng, {Hong Yun} and Jian Gu and Ying Xia and Mary McGrath and Sinisa Dovat and Chunhua Song and Yan Li",
year = "2018",
month = "6",
day = "1",
doi = "10.1016/j.leukres.2018.04.001",
language = "English (US)",
volume = "69",
pages = "47--53",
journal = "Leukemia Research",
issn = "0145-2126",
publisher = "Elsevier Limited",

}

New rapid method to detect BCR-ABL fusion genes with multiplex RT-qPCR in one-tube at a time. / Tong, Yong Qing; Zhao, Zhi Jun; Liu, Bei; Bao, An Yu; Zheng, Hong Yun; Gu, Jian; Xia, Ying; McGrath, Mary; Dovat, Sinisa; Song, Chunhua; Li, Yan.

In: Leukemia Research, Vol. 69, 01.06.2018, p. 47-53.

Research output: Contribution to journalArticle

TY - JOUR

T1 - New rapid method to detect BCR-ABL fusion genes with multiplex RT-qPCR in one-tube at a time

AU - Tong, Yong Qing

AU - Zhao, Zhi Jun

AU - Liu, Bei

AU - Bao, An Yu

AU - Zheng, Hong Yun

AU - Gu, Jian

AU - Xia, Ying

AU - McGrath, Mary

AU - Dovat, Sinisa

AU - Song, Chunhua

AU - Li, Yan

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Fast identification of BCR-ABL fusion genes is critical for precise diagnosis, risk stratification and therapy scheme selection in leukemia. More convenient methods are needed for quickly detection of the BCR-ABL fusion genes. Multiplex fluorescent reverse transcription quantitative real-time PCR (Multiplex RT-qPCR) methods are developed for detection of the at least 14 subtypes of BCR-ABL fusion genes in one tube at a time by using patients’ bone marrow samples. The new Multiplex RT-qPCR method could quickly detect BCR-ABL fusion genes with sensitivity up to 10–10 6 copies. It can detect the fusion genes in patients’ bone marrow samples containing any subtypes of the major bcr (M-bcr) e13a2, e14a2, e13a3 and e14a3, the minor bcr (m-bcr) e1a2 and e1a3, the micro bcr (μ-bcr) e19a2 and e19a3, and the nano bcr (n-bcr) e6a2 and e6a3. The specificity is comparable to the FISH methods. The cutoff for clinical diagnosis of BCR-ABL(+) is also determined by testing in clinical chronic myeloid leukemia samples. This is a new fast method with high sensitivity and specificity for clinical detection of BCR-ABL fusion genes. It will benefit the precise diagnosis, targeted therapy and minimal residual disease (MRD) monitoring in leukemia.

AB - Fast identification of BCR-ABL fusion genes is critical for precise diagnosis, risk stratification and therapy scheme selection in leukemia. More convenient methods are needed for quickly detection of the BCR-ABL fusion genes. Multiplex fluorescent reverse transcription quantitative real-time PCR (Multiplex RT-qPCR) methods are developed for detection of the at least 14 subtypes of BCR-ABL fusion genes in one tube at a time by using patients’ bone marrow samples. The new Multiplex RT-qPCR method could quickly detect BCR-ABL fusion genes with sensitivity up to 10–10 6 copies. It can detect the fusion genes in patients’ bone marrow samples containing any subtypes of the major bcr (M-bcr) e13a2, e14a2, e13a3 and e14a3, the minor bcr (m-bcr) e1a2 and e1a3, the micro bcr (μ-bcr) e19a2 and e19a3, and the nano bcr (n-bcr) e6a2 and e6a3. The specificity is comparable to the FISH methods. The cutoff for clinical diagnosis of BCR-ABL(+) is also determined by testing in clinical chronic myeloid leukemia samples. This is a new fast method with high sensitivity and specificity for clinical detection of BCR-ABL fusion genes. It will benefit the precise diagnosis, targeted therapy and minimal residual disease (MRD) monitoring in leukemia.

UR - http://www.scopus.com/inward/record.url?scp=85045195126&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045195126&partnerID=8YFLogxK

U2 - 10.1016/j.leukres.2018.04.001

DO - 10.1016/j.leukres.2018.04.001

M3 - Article

VL - 69

SP - 47

EP - 53

JO - Leukemia Research

JF - Leukemia Research

SN - 0145-2126

ER -