NF-κB transcription factors are involved in normal erythropoiesis

Min Ying Zhang, Shao Cong Sun, Laurie Bell, Barbara Miller

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

NF-κB/Rel designates a widely distributed family of transcription factors involved in immune and acute phase responses. Here, the expression and function of NF-κB factors in erythroid proliferation and differentiation were explored. In an erythroleukemia cell line, TF-1, high levels of p105/p50, p100/p52, p65, and IκBα were detected 24 hours after growth factor deprivation. In response to granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulation, significant induction of p52 expression was observed. GM-CSF also induced nuclear translocation of both p52 and p65. No induction of NF-κB factors was observed with erythropoietin stimulation of TF-1 cells. Overexpression of p52 and p65 in TF-1 cells by transient transfection resulted in significant induction of a κB-TATA-luciferase reporter plasmid, showing that these factors are functional in vivo in erythroid cells. To determine whether NF-κB factors may play a role in normal erythropoiesis, levels of these factors were determined in burst- forming unit-erythroid (BFU-E)derived cells at different stages of differentiation. The NF-κB factors p105/p50, p100/p52, and p65 were highly expressed in early BFU-E-derived precursors, which are rapidly proliferating, and declined during maturation. Furthermore, nuclear levels of NF-κB factors p50, p52, and p65 were higher in less mature precursors (day 10 BFU-E- derived cells) compared with more differentiated (day 14) erythroblasts. In nuclear extracts from day 10 BFU-E-derived cells, p50, p52, and p65 were able to form complexes, which bound to κB sites in the promoters of both the c- myb and c-myc genes, suggesting that c-myb and c-myc may be among the κB- containing genes regulated by NF-κB factors in normal erythroid cells. Taken together, these data show that NF-κB factors are modulated by GM-CSF and suggest they function to regulate specific KB containing genes involved in erythropoiesis.

Original languageEnglish (US)
Pages (from-to)4136-4144
Number of pages9
JournalBlood
Volume91
Issue number11
StatePublished - Jun 1 1998

Fingerprint

Erythropoiesis
Erythroid Cells
Transcription Factors
Granulocyte-Macrophage Colony-Stimulating Factor
Genes
Erythroblasts
Leukemia, Erythroblastic, Acute
Erythroid Precursor Cells
Acute-Phase Reaction
myc Genes
Erythropoietin
3'-(1-butylphosphoryl)adenosine
Luciferases
Transfection
Intercellular Signaling Peptides and Proteins
Plasmids
Cells
Cell Line

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Zhang, M. Y., Sun, S. C., Bell, L., & Miller, B. (1998). NF-κB transcription factors are involved in normal erythropoiesis. Blood, 91(11), 4136-4144.
Zhang, Min Ying ; Sun, Shao Cong ; Bell, Laurie ; Miller, Barbara. / NF-κB transcription factors are involved in normal erythropoiesis. In: Blood. 1998 ; Vol. 91, No. 11. pp. 4136-4144.
@article{946a9909029243128903e560aff0e27e,
title = "NF-κB transcription factors are involved in normal erythropoiesis",
abstract = "NF-κB/Rel designates a widely distributed family of transcription factors involved in immune and acute phase responses. Here, the expression and function of NF-κB factors in erythroid proliferation and differentiation were explored. In an erythroleukemia cell line, TF-1, high levels of p105/p50, p100/p52, p65, and IκBα were detected 24 hours after growth factor deprivation. In response to granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulation, significant induction of p52 expression was observed. GM-CSF also induced nuclear translocation of both p52 and p65. No induction of NF-κB factors was observed with erythropoietin stimulation of TF-1 cells. Overexpression of p52 and p65 in TF-1 cells by transient transfection resulted in significant induction of a κB-TATA-luciferase reporter plasmid, showing that these factors are functional in vivo in erythroid cells. To determine whether NF-κB factors may play a role in normal erythropoiesis, levels of these factors were determined in burst- forming unit-erythroid (BFU-E)derived cells at different stages of differentiation. The NF-κB factors p105/p50, p100/p52, and p65 were highly expressed in early BFU-E-derived precursors, which are rapidly proliferating, and declined during maturation. Furthermore, nuclear levels of NF-κB factors p50, p52, and p65 were higher in less mature precursors (day 10 BFU-E- derived cells) compared with more differentiated (day 14) erythroblasts. In nuclear extracts from day 10 BFU-E-derived cells, p50, p52, and p65 were able to form complexes, which bound to κB sites in the promoters of both the c- myb and c-myc genes, suggesting that c-myb and c-myc may be among the κB- containing genes regulated by NF-κB factors in normal erythroid cells. Taken together, these data show that NF-κB factors are modulated by GM-CSF and suggest they function to regulate specific KB containing genes involved in erythropoiesis.",
author = "Zhang, {Min Ying} and Sun, {Shao Cong} and Laurie Bell and Barbara Miller",
year = "1998",
month = "6",
day = "1",
language = "English (US)",
volume = "91",
pages = "4136--4144",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "11",

}

Zhang, MY, Sun, SC, Bell, L & Miller, B 1998, 'NF-κB transcription factors are involved in normal erythropoiesis', Blood, vol. 91, no. 11, pp. 4136-4144.

NF-κB transcription factors are involved in normal erythropoiesis. / Zhang, Min Ying; Sun, Shao Cong; Bell, Laurie; Miller, Barbara.

In: Blood, Vol. 91, No. 11, 01.06.1998, p. 4136-4144.

Research output: Contribution to journalArticle

TY - JOUR

T1 - NF-κB transcription factors are involved in normal erythropoiesis

AU - Zhang, Min Ying

AU - Sun, Shao Cong

AU - Bell, Laurie

AU - Miller, Barbara

PY - 1998/6/1

Y1 - 1998/6/1

N2 - NF-κB/Rel designates a widely distributed family of transcription factors involved in immune and acute phase responses. Here, the expression and function of NF-κB factors in erythroid proliferation and differentiation were explored. In an erythroleukemia cell line, TF-1, high levels of p105/p50, p100/p52, p65, and IκBα were detected 24 hours after growth factor deprivation. In response to granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulation, significant induction of p52 expression was observed. GM-CSF also induced nuclear translocation of both p52 and p65. No induction of NF-κB factors was observed with erythropoietin stimulation of TF-1 cells. Overexpression of p52 and p65 in TF-1 cells by transient transfection resulted in significant induction of a κB-TATA-luciferase reporter plasmid, showing that these factors are functional in vivo in erythroid cells. To determine whether NF-κB factors may play a role in normal erythropoiesis, levels of these factors were determined in burst- forming unit-erythroid (BFU-E)derived cells at different stages of differentiation. The NF-κB factors p105/p50, p100/p52, and p65 were highly expressed in early BFU-E-derived precursors, which are rapidly proliferating, and declined during maturation. Furthermore, nuclear levels of NF-κB factors p50, p52, and p65 were higher in less mature precursors (day 10 BFU-E- derived cells) compared with more differentiated (day 14) erythroblasts. In nuclear extracts from day 10 BFU-E-derived cells, p50, p52, and p65 were able to form complexes, which bound to κB sites in the promoters of both the c- myb and c-myc genes, suggesting that c-myb and c-myc may be among the κB- containing genes regulated by NF-κB factors in normal erythroid cells. Taken together, these data show that NF-κB factors are modulated by GM-CSF and suggest they function to regulate specific KB containing genes involved in erythropoiesis.

AB - NF-κB/Rel designates a widely distributed family of transcription factors involved in immune and acute phase responses. Here, the expression and function of NF-κB factors in erythroid proliferation and differentiation were explored. In an erythroleukemia cell line, TF-1, high levels of p105/p50, p100/p52, p65, and IκBα were detected 24 hours after growth factor deprivation. In response to granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulation, significant induction of p52 expression was observed. GM-CSF also induced nuclear translocation of both p52 and p65. No induction of NF-κB factors was observed with erythropoietin stimulation of TF-1 cells. Overexpression of p52 and p65 in TF-1 cells by transient transfection resulted in significant induction of a κB-TATA-luciferase reporter plasmid, showing that these factors are functional in vivo in erythroid cells. To determine whether NF-κB factors may play a role in normal erythropoiesis, levels of these factors were determined in burst- forming unit-erythroid (BFU-E)derived cells at different stages of differentiation. The NF-κB factors p105/p50, p100/p52, and p65 were highly expressed in early BFU-E-derived precursors, which are rapidly proliferating, and declined during maturation. Furthermore, nuclear levels of NF-κB factors p50, p52, and p65 were higher in less mature precursors (day 10 BFU-E- derived cells) compared with more differentiated (day 14) erythroblasts. In nuclear extracts from day 10 BFU-E-derived cells, p50, p52, and p65 were able to form complexes, which bound to κB sites in the promoters of both the c- myb and c-myc genes, suggesting that c-myb and c-myc may be among the κB- containing genes regulated by NF-κB factors in normal erythroid cells. Taken together, these data show that NF-κB factors are modulated by GM-CSF and suggest they function to regulate specific KB containing genes involved in erythropoiesis.

UR - http://www.scopus.com/inward/record.url?scp=0032081311&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032081311&partnerID=8YFLogxK

M3 - Article

C2 - 9596659

AN - SCOPUS:0032081311

VL - 91

SP - 4136

EP - 4144

JO - Blood

JF - Blood

SN - 0006-4971

IS - 11

ER -

Zhang MY, Sun SC, Bell L, Miller B. NF-κB transcription factors are involved in normal erythropoiesis. Blood. 1998 Jun 1;91(11):4136-4144.