Nitrofen dose-dependent gestational day-specific murine lung hypoplasia and left-sided diaphragmatic hernia

Robert Cilley, Steven E. Zgleszewski, Thomas M. Krummel, Mala R. Chinoy

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

2,4-Dichlorophenyl-p-nitrophenyl ether (nitrofen) is known to induce pulmonary hypoplasia (PH) with or without diaphragmatic hernias (DH) in rats and mice. We determined the timing of administration and dose of nitrofen needed to create left-sided DH and PH in fetal mice. Time-dated pregnant CD- 1 mice were gavaged with various doses of nitrofen in the later one-half of gestational days (GD) 8-11. Fetuses were removed by laparotomy at GD 14, fixed, and evaluated histologically. Fetal lung size was inversely related to nitrofen dose. Morphometric analysis of normal and nitrofen-exposed hypoplastic lungs at the pseudoglandular stage revealed significant differences in lung length, surface area, and in the number of airways. Left- sided DH were observed in a 'dorsolateral' position accompanied by PH in ~30% of GD 14 fetuses exposed to 25 mg nitrofen on GD 8. A minimal portion of liver was present in the hernia. The lungs of fetuses exposed on GD 9, 10, and 11 progressed to near normal size. Murine fetuses exposed to 25 mg nitrofen on GD 8 resulted in PH and DH, whereas other doses created dose- dependent PH alone or none at all on GD 11. Our study established that, to create left-sided DH and PH in murine fetuses, nitrofen dose specificity and time of administration during gestation were crucial.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume272
Issue number2 16-2
StatePublished - Feb 1 1997

Fingerprint

Diaphragmatic Hernia
Lung
Fetus
nitrofen
Hernia
Ether
Laparotomy
Pregnancy

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

@article{bd1c40fbb3e44a528d51a7af2e778a79,
title = "Nitrofen dose-dependent gestational day-specific murine lung hypoplasia and left-sided diaphragmatic hernia",
abstract = "2,4-Dichlorophenyl-p-nitrophenyl ether (nitrofen) is known to induce pulmonary hypoplasia (PH) with or without diaphragmatic hernias (DH) in rats and mice. We determined the timing of administration and dose of nitrofen needed to create left-sided DH and PH in fetal mice. Time-dated pregnant CD- 1 mice were gavaged with various doses of nitrofen in the later one-half of gestational days (GD) 8-11. Fetuses were removed by laparotomy at GD 14, fixed, and evaluated histologically. Fetal lung size was inversely related to nitrofen dose. Morphometric analysis of normal and nitrofen-exposed hypoplastic lungs at the pseudoglandular stage revealed significant differences in lung length, surface area, and in the number of airways. Left- sided DH were observed in a 'dorsolateral' position accompanied by PH in ~30{\%} of GD 14 fetuses exposed to 25 mg nitrofen on GD 8. A minimal portion of liver was present in the hernia. The lungs of fetuses exposed on GD 9, 10, and 11 progressed to near normal size. Murine fetuses exposed to 25 mg nitrofen on GD 8 resulted in PH and DH, whereas other doses created dose- dependent PH alone or none at all on GD 11. Our study established that, to create left-sided DH and PH in murine fetuses, nitrofen dose specificity and time of administration during gestation were crucial.",
author = "Robert Cilley and Zgleszewski, {Steven E.} and Krummel, {Thomas M.} and Chinoy, {Mala R.}",
year = "1997",
month = "2",
day = "1",
language = "English (US)",
volume = "272",
journal = "American Journal of Physiology",
issn = "1040-0605",
publisher = "American Physiological Society",
number = "2 16-2",

}

Nitrofen dose-dependent gestational day-specific murine lung hypoplasia and left-sided diaphragmatic hernia. / Cilley, Robert; Zgleszewski, Steven E.; Krummel, Thomas M.; Chinoy, Mala R.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 272, No. 2 16-2, 01.02.1997.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nitrofen dose-dependent gestational day-specific murine lung hypoplasia and left-sided diaphragmatic hernia

AU - Cilley, Robert

AU - Zgleszewski, Steven E.

AU - Krummel, Thomas M.

AU - Chinoy, Mala R.

PY - 1997/2/1

Y1 - 1997/2/1

N2 - 2,4-Dichlorophenyl-p-nitrophenyl ether (nitrofen) is known to induce pulmonary hypoplasia (PH) with or without diaphragmatic hernias (DH) in rats and mice. We determined the timing of administration and dose of nitrofen needed to create left-sided DH and PH in fetal mice. Time-dated pregnant CD- 1 mice were gavaged with various doses of nitrofen in the later one-half of gestational days (GD) 8-11. Fetuses were removed by laparotomy at GD 14, fixed, and evaluated histologically. Fetal lung size was inversely related to nitrofen dose. Morphometric analysis of normal and nitrofen-exposed hypoplastic lungs at the pseudoglandular stage revealed significant differences in lung length, surface area, and in the number of airways. Left- sided DH were observed in a 'dorsolateral' position accompanied by PH in ~30% of GD 14 fetuses exposed to 25 mg nitrofen on GD 8. A minimal portion of liver was present in the hernia. The lungs of fetuses exposed on GD 9, 10, and 11 progressed to near normal size. Murine fetuses exposed to 25 mg nitrofen on GD 8 resulted in PH and DH, whereas other doses created dose- dependent PH alone or none at all on GD 11. Our study established that, to create left-sided DH and PH in murine fetuses, nitrofen dose specificity and time of administration during gestation were crucial.

AB - 2,4-Dichlorophenyl-p-nitrophenyl ether (nitrofen) is known to induce pulmonary hypoplasia (PH) with or without diaphragmatic hernias (DH) in rats and mice. We determined the timing of administration and dose of nitrofen needed to create left-sided DH and PH in fetal mice. Time-dated pregnant CD- 1 mice were gavaged with various doses of nitrofen in the later one-half of gestational days (GD) 8-11. Fetuses were removed by laparotomy at GD 14, fixed, and evaluated histologically. Fetal lung size was inversely related to nitrofen dose. Morphometric analysis of normal and nitrofen-exposed hypoplastic lungs at the pseudoglandular stage revealed significant differences in lung length, surface area, and in the number of airways. Left- sided DH were observed in a 'dorsolateral' position accompanied by PH in ~30% of GD 14 fetuses exposed to 25 mg nitrofen on GD 8. A minimal portion of liver was present in the hernia. The lungs of fetuses exposed on GD 9, 10, and 11 progressed to near normal size. Murine fetuses exposed to 25 mg nitrofen on GD 8 resulted in PH and DH, whereas other doses created dose- dependent PH alone or none at all on GD 11. Our study established that, to create left-sided DH and PH in murine fetuses, nitrofen dose specificity and time of administration during gestation were crucial.

UR - http://www.scopus.com/inward/record.url?scp=0030909972&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030909972&partnerID=8YFLogxK

M3 - Article

VL - 272

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 1040-0605

IS - 2 16-2

ER -