NMR-Based Metabolomics and Its Application in Drug Metabolism and Cancer Research

Limin Zhang, Emmanuel Hatzakis, Andrew David Patterson

Research output: Contribution to journalReview article

4 Citations (Scopus)

Abstract

Purpose of Review: In this review, the contribution of NMR to the metabolomics field will be discussed—particularly as it relates to cancer and drug metabolism research. An overview of the typical NMR metabolomic experiment will be presented while emphasizing important caveats ranging from experimental design to data interpretation. Recent Findings: Despite some of the drawbacks of NMR, including reduced sensitivity compared to mass spectrometry-based approaches, NMR-based metabolomic approaches have provided considerable insight into drug and cancer metabolism. Advances in flux analysis based on the strategic use of isotopes have generated unique perspectives of cancer from the cellular and tissue levels. Summary: Combined with other “omics” tools, including genomics, proteomics, and transcriptomics, metabolomic analysis completes the holistic view of metabolism. Many of these NMR-based approaches are likely to find their way into the clinic and become a common diagnostic tool.

Original languageEnglish (US)
Pages (from-to)231-240
Number of pages10
JournalCurrent Pharmacology Reports
Volume2
Issue number5
DOIs
StatePublished - Oct 1 2016

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Metabolomics
Metabolism
Nuclear magnetic resonance
Research
Pharmaceutical Preparations
Neoplasms
Genomics
Isotopes
Proteomics
Mass Spectrometry
Research Design
Design of experiments
Mass spectrometry
Tissue
Fluxes
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Genetics
  • Pharmacology
  • Drug Discovery

Cite this

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abstract = "Purpose of Review: In this review, the contribution of NMR to the metabolomics field will be discussed—particularly as it relates to cancer and drug metabolism research. An overview of the typical NMR metabolomic experiment will be presented while emphasizing important caveats ranging from experimental design to data interpretation. Recent Findings: Despite some of the drawbacks of NMR, including reduced sensitivity compared to mass spectrometry-based approaches, NMR-based metabolomic approaches have provided considerable insight into drug and cancer metabolism. Advances in flux analysis based on the strategic use of isotopes have generated unique perspectives of cancer from the cellular and tissue levels. Summary: Combined with other “omics” tools, including genomics, proteomics, and transcriptomics, metabolomic analysis completes the holistic view of metabolism. Many of these NMR-based approaches are likely to find their way into the clinic and become a common diagnostic tool.",
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NMR-Based Metabolomics and Its Application in Drug Metabolism and Cancer Research. / Zhang, Limin; Hatzakis, Emmanuel; Patterson, Andrew David.

In: Current Pharmacology Reports, Vol. 2, No. 5, 01.10.2016, p. 231-240.

Research output: Contribution to journalReview article

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