No association between body mass index and β3-adrenergic receptor variant (W64R) in children with premature pubarche and adolescent girls with hyperandrogenism

Selma F. Witchel, Julian Fagerli, Jessica Siegel, Rhonda Smith, Mohamed Farouk Mitwally, Vered Lewy, Silva Arslanian, Peter Lee

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective: To determine if the Trp64Arg (W64R) variant of the β3- adrenergic receptor (ADRB3) could be used as a genetic marker to define risk for polycystic ovary syndrom (PCOS) and/or obesity in children and adolescents. Design: Association study. Setting: Academic research environment. Patient(s): Children referred for evaluation of premature pubic hair (n = 63), adolescent girls referred for evaluation of hirsutism and/or oligomenorrhea (n = 33), and healthy adult controls (n = 67). Intervention(s): None. Main Outcome Measure(s): Relationship of body mass index (BMI) to presence or absence of W64R variant and frequency of W64R variant in our patient population. Result(s): Body mass index (kg/m2) was determined for 63 children (55 girls and 8 boys) and 33 adolescent girls. Presence or absence of the W64R variant was assayed by polymerase chain reaction (PCR) amplification followed by allele-specific restriction fragment digest. Twelve subjects and 11 healthy controls were found to be heterozygous for the W64R variant. One subject was found to be homozygous for the W64R variant. Allele frequency for the W64R variant was comparable between patients and controls. Among the patients, mean BMI values were not different between carriers and noncarriers. Conclusion(s): Although other studies suggest that the W64R variant is associated with the development of obesity and insulin resistance, we cannot demonstrate that it has a major effect on BMI in children with premature pubarche or in adolescent girls with hyperandrogenism. Serial observations are necessary to determine if this variant predicts the development of obesity and/or PCOS in adulthood. (C)2000 by American Society for Reproductive Medicine.

Original languageEnglish (US)
Pages (from-to)509-515
Number of pages7
JournalFertility and sterility
Volume73
Issue number3
DOIs
StatePublished - Mar 1 2000

Fingerprint

Hyperandrogenism
Adrenergic Receptors
Body Mass Index
Pediatric Obesity
Ovary
Obesity
Oligomenorrhea
Hirsutism
Genetic Markers
Gene Frequency
Hair
Insulin Resistance
Healthy Volunteers
Alleles
Outcome Assessment (Health Care)
Polymerase Chain Reaction
Research
Population

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Witchel, Selma F. ; Fagerli, Julian ; Siegel, Jessica ; Smith, Rhonda ; Mitwally, Mohamed Farouk ; Lewy, Vered ; Arslanian, Silva ; Lee, Peter. / No association between body mass index and β3-adrenergic receptor variant (W64R) in children with premature pubarche and adolescent girls with hyperandrogenism. In: Fertility and sterility. 2000 ; Vol. 73, No. 3. pp. 509-515.
@article{c41b554068894328983eda5dc4e04fee,
title = "No association between body mass index and β3-adrenergic receptor variant (W64R) in children with premature pubarche and adolescent girls with hyperandrogenism",
abstract = "Objective: To determine if the Trp64Arg (W64R) variant of the β3- adrenergic receptor (ADRB3) could be used as a genetic marker to define risk for polycystic ovary syndrom (PCOS) and/or obesity in children and adolescents. Design: Association study. Setting: Academic research environment. Patient(s): Children referred for evaluation of premature pubic hair (n = 63), adolescent girls referred for evaluation of hirsutism and/or oligomenorrhea (n = 33), and healthy adult controls (n = 67). Intervention(s): None. Main Outcome Measure(s): Relationship of body mass index (BMI) to presence or absence of W64R variant and frequency of W64R variant in our patient population. Result(s): Body mass index (kg/m2) was determined for 63 children (55 girls and 8 boys) and 33 adolescent girls. Presence or absence of the W64R variant was assayed by polymerase chain reaction (PCR) amplification followed by allele-specific restriction fragment digest. Twelve subjects and 11 healthy controls were found to be heterozygous for the W64R variant. One subject was found to be homozygous for the W64R variant. Allele frequency for the W64R variant was comparable between patients and controls. Among the patients, mean BMI values were not different between carriers and noncarriers. Conclusion(s): Although other studies suggest that the W64R variant is associated with the development of obesity and insulin resistance, we cannot demonstrate that it has a major effect on BMI in children with premature pubarche or in adolescent girls with hyperandrogenism. Serial observations are necessary to determine if this variant predicts the development of obesity and/or PCOS in adulthood. (C)2000 by American Society for Reproductive Medicine.",
author = "Witchel, {Selma F.} and Julian Fagerli and Jessica Siegel and Rhonda Smith and Mitwally, {Mohamed Farouk} and Vered Lewy and Silva Arslanian and Peter Lee",
year = "2000",
month = "3",
day = "1",
doi = "10.1016/S0015-0282(99)00568-3",
language = "English (US)",
volume = "73",
pages = "509--515",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "3",

}

No association between body mass index and β3-adrenergic receptor variant (W64R) in children with premature pubarche and adolescent girls with hyperandrogenism. / Witchel, Selma F.; Fagerli, Julian; Siegel, Jessica; Smith, Rhonda; Mitwally, Mohamed Farouk; Lewy, Vered; Arslanian, Silva; Lee, Peter.

In: Fertility and sterility, Vol. 73, No. 3, 01.03.2000, p. 509-515.

Research output: Contribution to journalArticle

TY - JOUR

T1 - No association between body mass index and β3-adrenergic receptor variant (W64R) in children with premature pubarche and adolescent girls with hyperandrogenism

AU - Witchel, Selma F.

AU - Fagerli, Julian

AU - Siegel, Jessica

AU - Smith, Rhonda

AU - Mitwally, Mohamed Farouk

AU - Lewy, Vered

AU - Arslanian, Silva

AU - Lee, Peter

PY - 2000/3/1

Y1 - 2000/3/1

N2 - Objective: To determine if the Trp64Arg (W64R) variant of the β3- adrenergic receptor (ADRB3) could be used as a genetic marker to define risk for polycystic ovary syndrom (PCOS) and/or obesity in children and adolescents. Design: Association study. Setting: Academic research environment. Patient(s): Children referred for evaluation of premature pubic hair (n = 63), adolescent girls referred for evaluation of hirsutism and/or oligomenorrhea (n = 33), and healthy adult controls (n = 67). Intervention(s): None. Main Outcome Measure(s): Relationship of body mass index (BMI) to presence or absence of W64R variant and frequency of W64R variant in our patient population. Result(s): Body mass index (kg/m2) was determined for 63 children (55 girls and 8 boys) and 33 adolescent girls. Presence or absence of the W64R variant was assayed by polymerase chain reaction (PCR) amplification followed by allele-specific restriction fragment digest. Twelve subjects and 11 healthy controls were found to be heterozygous for the W64R variant. One subject was found to be homozygous for the W64R variant. Allele frequency for the W64R variant was comparable between patients and controls. Among the patients, mean BMI values were not different between carriers and noncarriers. Conclusion(s): Although other studies suggest that the W64R variant is associated with the development of obesity and insulin resistance, we cannot demonstrate that it has a major effect on BMI in children with premature pubarche or in adolescent girls with hyperandrogenism. Serial observations are necessary to determine if this variant predicts the development of obesity and/or PCOS in adulthood. (C)2000 by American Society for Reproductive Medicine.

AB - Objective: To determine if the Trp64Arg (W64R) variant of the β3- adrenergic receptor (ADRB3) could be used as a genetic marker to define risk for polycystic ovary syndrom (PCOS) and/or obesity in children and adolescents. Design: Association study. Setting: Academic research environment. Patient(s): Children referred for evaluation of premature pubic hair (n = 63), adolescent girls referred for evaluation of hirsutism and/or oligomenorrhea (n = 33), and healthy adult controls (n = 67). Intervention(s): None. Main Outcome Measure(s): Relationship of body mass index (BMI) to presence or absence of W64R variant and frequency of W64R variant in our patient population. Result(s): Body mass index (kg/m2) was determined for 63 children (55 girls and 8 boys) and 33 adolescent girls. Presence or absence of the W64R variant was assayed by polymerase chain reaction (PCR) amplification followed by allele-specific restriction fragment digest. Twelve subjects and 11 healthy controls were found to be heterozygous for the W64R variant. One subject was found to be homozygous for the W64R variant. Allele frequency for the W64R variant was comparable between patients and controls. Among the patients, mean BMI values were not different between carriers and noncarriers. Conclusion(s): Although other studies suggest that the W64R variant is associated with the development of obesity and insulin resistance, we cannot demonstrate that it has a major effect on BMI in children with premature pubarche or in adolescent girls with hyperandrogenism. Serial observations are necessary to determine if this variant predicts the development of obesity and/or PCOS in adulthood. (C)2000 by American Society for Reproductive Medicine.

UR - http://www.scopus.com/inward/record.url?scp=0033954924&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033954924&partnerID=8YFLogxK

U2 - 10.1016/S0015-0282(99)00568-3

DO - 10.1016/S0015-0282(99)00568-3

M3 - Article

C2 - 10689004

AN - SCOPUS:0033954924

VL - 73

SP - 509

EP - 515

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 3

ER -