Non-lethal method of DNA sampling in euglossine bees supported by mark-recapture experiments and microsatellite genotyping

Cíntia Akemi Oi, Margarita M. López-Uribe, Marcelo Cervini, Marco Antonio Del Lama

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Non-lethal sampling methods are of great interest for conservation genetic studies to prevent the death of individuals in populations that are threatened or in decline. With this aim, we tested a non-lethal method of partial antennae removal for DNA sampling in two euglossine bee species: Euglossa cordata and Eulaema nigrita. We validated the survival of the individuals through mark-recapture experiments during 16 months. The quality and quantity of the tissue for DNA analysis was verified through amplification and genotyping of nine and eleven microsatellite loci, respectively. Our results from the mark-recapture experiments showed equal recapture rates of individuals with intact and removed antennae (E. cordata χ2 = 2.492, df = 1, p = 0.114; E. nigrita χ2 = 1.683, df = 1, p = 0.194). Microsatellite loci were successfully genotyped in 97.1 and 97.6 % of the E. cordata and E. nigrita individuals, respectively. Our results validate the feasibility of using antennae tissue for DNA genetic analysis without compromising the survival of individual bees.

Original languageEnglish (US)
Pages (from-to)1071-1079
Number of pages9
JournalJournal of Insect Conservation
Volume17
Issue number5
DOIs
StatePublished - Oct 1 2013

All Science Journal Classification (ASJC) codes

  • Ecology
  • Animal Science and Zoology
  • Nature and Landscape Conservation
  • Insect Science

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