Noncoding mitochondrial loci for corals

Gregory T. Concepcion, Monica Medina, Robert J. Toonen

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

We developed five degenerate primer pairs for the amplification and sequencing of two noncoding regions found in the mitochondrial genome of corals. These primers amplify products ranging from 380 to 950 bp, and work in a wide variety of scleractinian taxa from both the Pacific and Caribbean. Based on our initial analysis of ∼300 sequences from 13 scleractinian taxa, both these noncoding regions appear to have equivalent levels of variability to the most variable of previously published coral mitochondrial loci, but work in a wider variety of taxa. We believe these primers will be of use to coral biologists studying questions above the level of species; as with other mithochondrial DNA markers in corals, these loci will likely provide little resolution for within-species studies.

Original languageEnglish (US)
Pages (from-to)1208-1211
Number of pages4
JournalMolecular Ecology Notes
Volume6
Issue number4
DOIs
StatePublished - Dec 1 2006

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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