Nonfunctional epidermal growth factor receptor in cells transformed by Kirsten sarcoma virus

Chu Chang Chua, Deborah E. Geiman, Alain B. Schreiber, Roger Ladda

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The cell membrane receptor for epidermal growth factor (EGF) appears to be a glycoprotein of Mr 170,000 and mediates the mitogenic and metabolic responses of cells with EGF receptors (EGF-R). Normal rat kidney (NRK) have about 3 × 105 EGF-R per cell. Upon transformation of NRK cells by Kirsten sarcoma virus, the transformed derivative (KNRK) loses the ability to bind 125I-EGF. Membranes from NRK and KNRK cells were included in EGF-dependent phosphorylation reactions to search for evidence of the EGF-R. A phosphorylated protein of Mr 170,000 was detected in both NRK and KNRK membranes. The Mr 170,000 protein was identified to be EGF-R by immunoprecipitation with monoclonal antibody to the receptor. Furthermore, two-dimensional peptide mapping using trypsin and chymotrypsin digestions of the iodinated receptors from both NRK and KNRK cells showed essentially identical patterns. These data indicate that the EGF-R is present in KNRK cells with apparently the same protein structure as the NRK counterpart.

Original languageEnglish (US)
Pages (from-to)538-547
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume118
Issue number2
DOIs
StatePublished - Jan 30 1984

Fingerprint

Kirsten murine sarcoma virus
Viruses
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Rats
Kidney
Membranes
Phosphorylation
Proteins
Peptide Mapping
Cell membranes
Immunoprecipitation
Digestion
Glycoproteins
Monoclonal Antibodies
Cell Membrane
Derivatives
Peptides

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Chua, Chu Chang ; Geiman, Deborah E. ; Schreiber, Alain B. ; Ladda, Roger. / Nonfunctional epidermal growth factor receptor in cells transformed by Kirsten sarcoma virus. In: Biochemical and Biophysical Research Communications. 1984 ; Vol. 118, No. 2. pp. 538-547.
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Nonfunctional epidermal growth factor receptor in cells transformed by Kirsten sarcoma virus. / Chua, Chu Chang; Geiman, Deborah E.; Schreiber, Alain B.; Ladda, Roger.

In: Biochemical and Biophysical Research Communications, Vol. 118, No. 2, 30.01.1984, p. 538-547.

Research output: Contribution to journalArticle

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