Nonmuscle myosin heavy chain B is recognized by a monoclonal antibody that inhibits GM1-enhanced neuritogenesis

Christine M. Mummert, Cara-Lynne Schengrund

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Addition of gangliosides to neuroblastoma cells maintained in vitro has been shown to enhance neuritogenesis. Although the mechanism by which they exert this effect is unknown, it has been postulated that they may act by adhering to cell surface proteins. In this article, we describe the isolation and identification of an S20Y murine neuroblastoma cell protein recognized by a monoclonal antibody that was prepared against putative GM1-binding proteins and shown to inhibit GM1-enhanced neuritogenesis. The protein identified was nonmuscle myosin heavy chain B, which appears to function in neurite formation but may not adhere to gangliosides.

Original languageEnglish (US)
Pages (from-to)596-600
Number of pages5
JournalJournal of Neurochemistry
Volume68
Issue number2
StatePublished - Feb 1 1997

Fingerprint

Gangliosides
Neuroblastoma
Monoclonal Antibodies
Neurites
Carrier Proteins
Membrane Proteins
Proteins
nonmuscle myosin type IIB heavy chain
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

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abstract = "Addition of gangliosides to neuroblastoma cells maintained in vitro has been shown to enhance neuritogenesis. Although the mechanism by which they exert this effect is unknown, it has been postulated that they may act by adhering to cell surface proteins. In this article, we describe the isolation and identification of an S20Y murine neuroblastoma cell protein recognized by a monoclonal antibody that was prepared against putative GM1-binding proteins and shown to inhibit GM1-enhanced neuritogenesis. The protein identified was nonmuscle myosin heavy chain B, which appears to function in neurite formation but may not adhere to gangliosides.",
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Nonmuscle myosin heavy chain B is recognized by a monoclonal antibody that inhibits GM1-enhanced neuritogenesis. / Mummert, Christine M.; Schengrund, Cara-Lynne.

In: Journal of Neurochemistry, Vol. 68, No. 2, 01.02.1997, p. 596-600.

Research output: Contribution to journalArticle

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