Nonmuscle myosin II localizes to the Z-lines and intercalated discs of cardiac muscle and to the Z-lines of skeletal muscle

Kazuyo Takeda, Zu Xi Yu, Sujuan Qian, Thomas Chin, Robert S. Adelstein, Victor J. Ferrans

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

To understand the role of nonmuscle myosin II in cardiac and skeletal muscle, we used a number of polyclonal antibodies, three detecting nonmuscle myosin heavy chain II-B (NMHC II-B) and two detecting NMHC II-A, to examine the localization of these two proteins in fresh-frozen, acetone-fixed sections of normal human and mouse hearts and human skeletal muscles. Results were similar in both species and were confirmed by examination of fresh- frozen sections of human hearts subjected to no fixation or to treatment with either 4% p-formaldehyde or 50% glycerol. NMHC II-B was diffusely distributed in the cytoplasm of cardiac myocytes during development, but after birth it was localized to the Z-lines and intercalated discs. Dual labeling showed almost complete colocalization of NMHC II-B with α-actinin. Whereas endothelial cells, smooth muscle cells and fibroblasts showed strong immunoreactivity for NMHC II-A and NMHC II-B, cardiac myocytes only showed reactivity for the latter. The Z-lines of human skeletal muscle cells, in contrast to those of cardiac myocytes, gave positive reactions for both NMHC II-A and NMHC II-B. The presence of a motor protein in the Z-lines and intercalated discs raises the possibility that these structures may play a more dynamic role in the contraction/relaxation mechanism of cardiac and skeletal muscle than has been previously suspected. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)59-68
Number of pages10
JournalCell Motility and the Cytoskeleton
Volume46
Issue number1
DOIs
StatePublished - Jan 1 2000

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Myosin Type II
Myocardium
Skeletal Muscle
Cardiac Myocytes
Actinin
Frozen Sections
Acetone
Glycerol
Muscle Cells
Formaldehyde
Smooth Muscle Myocytes
Cytoplasm
Endothelial Cells
Fibroblasts
Parturition
nonmuscle myosin type IIB heavy chain
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Cell Biology

Cite this

Takeda, Kazuyo ; Yu, Zu Xi ; Qian, Sujuan ; Chin, Thomas ; Adelstein, Robert S. ; Ferrans, Victor J. / Nonmuscle myosin II localizes to the Z-lines and intercalated discs of cardiac muscle and to the Z-lines of skeletal muscle. In: Cell Motility and the Cytoskeleton. 2000 ; Vol. 46, No. 1. pp. 59-68.
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Nonmuscle myosin II localizes to the Z-lines and intercalated discs of cardiac muscle and to the Z-lines of skeletal muscle. / Takeda, Kazuyo; Yu, Zu Xi; Qian, Sujuan; Chin, Thomas; Adelstein, Robert S.; Ferrans, Victor J.

In: Cell Motility and the Cytoskeleton, Vol. 46, No. 1, 01.01.2000, p. 59-68.

Research output: Contribution to journalArticle

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