Nonspecific alcoholysis, a novel endonuclease activity of human immunodeficiency virus type 1 and other retroviral integrases

Michael Katzman, Malgorzata Sudol

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Retroviral integrase (IN) exhibits a previously unrecognized endonuclease activity which we have termed nonspecific alcoholysis. This action occurred at every position in nonviral DNA sequences except those near 5' ends and is clearly distinguished from, and was not predicted by, the site-specific alcoholysis activity previously described for IN at the processing site near viral DNA termini. The integrases of human immunodeficiency virus type 1, visna virus, and Rous sarcoma virus exhibited different target site preferences in this new assay. The isolated central domain of human immunodeficiency virus type 1 IN preferred the same sites as the full-length protein. Nonspecific alcoholysis may provide insights into the structure and function of IN and other endonucleases and suggests that stimulators of some activities possessed by retroviral enzymes should be sought as antiviral agents.

Original languageEnglish (US)
Pages (from-to)2598-2604
Number of pages7
JournalJournal of virology
Volume70
Issue number4
StatePublished - Mar 27 1996

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alcoholysis
Integrases
Endonucleases
Human immunodeficiency virus 1
HIV-1
Visna maedi virus
Rous sarcoma virus
antiviral agents
Visna-maedi virus
Viral DNA
Antiviral Agents
nucleotide sequences
DNA
assays
enzymes
Enzymes
proteins
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

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abstract = "Retroviral integrase (IN) exhibits a previously unrecognized endonuclease activity which we have termed nonspecific alcoholysis. This action occurred at every position in nonviral DNA sequences except those near 5' ends and is clearly distinguished from, and was not predicted by, the site-specific alcoholysis activity previously described for IN at the processing site near viral DNA termini. The integrases of human immunodeficiency virus type 1, visna virus, and Rous sarcoma virus exhibited different target site preferences in this new assay. The isolated central domain of human immunodeficiency virus type 1 IN preferred the same sites as the full-length protein. Nonspecific alcoholysis may provide insights into the structure and function of IN and other endonucleases and suggests that stimulators of some activities possessed by retroviral enzymes should be sought as antiviral agents.",
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Nonspecific alcoholysis, a novel endonuclease activity of human immunodeficiency virus type 1 and other retroviral integrases. / Katzman, Michael; Sudol, Malgorzata.

In: Journal of virology, Vol. 70, No. 4, 27.03.1996, p. 2598-2604.

Research output: Contribution to journalArticle

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