Novel anti-factor D antibody inhibits complement, neutrophil, and platelet activation in a simulated pediatric cardiopulmonary bypass circuit

Akif Undar, P. G. Loubser, M. Fung, M. Mueller, C. Sun, W. N. Sun, K. Pinkston, C. Peng, W. K. Vaughn, C. D. Fraser

Research output: Contribution to journalConference article

Abstract

A study using human blood in a simulated pediatric cardiopulmonary bypass (CPB) circuit was carried out to investigate the effects of an anti-factor D monoclonal antibody on inhibition of the alternative complement cascade. Five paired experiments were performed on Mab 166-32 and an irrelevant control Mab at 18 μg/ml of human blood. Activation of complement, neutrophils, and platelets was assessed with immunofluorocytometry and ELISA. Complement activation via the alternative complement pathway induced the inflammatory responses in CPB, which were effectively inhibited by the anti-factor D Mab 166-32.

Original languageEnglish (US)
Number of pages1
JournalASAIO Journal
Volume46
Issue number2
DOIs
StatePublished - Jan 1 2000
Event46th Annual Conference and Exposition of ASAIO - New York, NY, USA
Duration: Jun 28 2000Jul 1 2000

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Neutrophil Activation
Pediatrics
Complement Activation
Platelet Activation
Platelets
Cardiopulmonary Bypass
Antibodies
Blood
Chemical activation
Alternative Complement Pathway
Monoclonal antibodies
Networks (circuits)
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Experiments
RHO(D) antibody

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Undar, Akif ; Loubser, P. G. ; Fung, M. ; Mueller, M. ; Sun, C. ; Sun, W. N. ; Pinkston, K. ; Peng, C. ; Vaughn, W. K. ; Fraser, C. D. / Novel anti-factor D antibody inhibits complement, neutrophil, and platelet activation in a simulated pediatric cardiopulmonary bypass circuit. In: ASAIO Journal. 2000 ; Vol. 46, No. 2.
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Novel anti-factor D antibody inhibits complement, neutrophil, and platelet activation in a simulated pediatric cardiopulmonary bypass circuit. / Undar, Akif; Loubser, P. G.; Fung, M.; Mueller, M.; Sun, C.; Sun, W. N.; Pinkston, K.; Peng, C.; Vaughn, W. K.; Fraser, C. D.

In: ASAIO Journal, Vol. 46, No. 2, 01.01.2000.

Research output: Contribution to journalConference article

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T1 - Novel anti-factor D antibody inhibits complement, neutrophil, and platelet activation in a simulated pediatric cardiopulmonary bypass circuit

AU - Undar, Akif

AU - Loubser, P. G.

AU - Fung, M.

AU - Mueller, M.

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AU - Sun, W. N.

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