Novel infectious particles generated by expression of the vesicular stomatitis virus glycoprotein from a self-replicating RNA

Melissa Rolls, Paul Webster, Nader H. Balba, John K. Rose

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Self-propagating infectious particles were produced in animal cells transfected with an RNA replicon encoding a single viral structural protein, the vesicular stomatitis virus glycoprotein (VSV-G). The replicon is derived from an alphavirus, Semliki Forest virus (SFV), and encodes the SFV RNA replicase, but none of the SFV structural proteins. After transfection of the replicon into tissue culture cells, expression of G protein spread from small foci throughout the culture. Supernatants from the cells contained infectious, virus-like particles that could be passaged and were neutralized by anti-VSV serum. The majority of the infectious particles were smaller and less dense than either VSV or SFV. Characterization by electron microscopy showed membrane-enveloped vesicles that contained the VSV-G protein. Infectious particles were apparently generated by budding of vesicles containing VSV-G protein and the RNA replicon. These experiments reveal that an enveloped infectious agent can be much simpler than previously thought.

Original languageEnglish (US)
Pages (from-to)497-506
Number of pages10
JournalCell
Volume79
Issue number3
DOIs
StatePublished - Nov 4 1994

Fingerprint

Semliki forest virus
Replicon
Vesicular Stomatitis
Viruses
Glycoproteins
RNA
Viral Structural Proteins
Alphavirus
RNA Replicase
Monomeric GTP-Binding Proteins
Virion
Transfection
Electron Microscopy
Proteins
Cell Culture Techniques
Tissue culture
Membranes
Electron microscopy
Serum
Animals

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Molecular Biology

Cite this

Rolls, Melissa ; Webster, Paul ; Balba, Nader H. ; Rose, John K. / Novel infectious particles generated by expression of the vesicular stomatitis virus glycoprotein from a self-replicating RNA. In: Cell. 1994 ; Vol. 79, No. 3. pp. 497-506.
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Novel infectious particles generated by expression of the vesicular stomatitis virus glycoprotein from a self-replicating RNA. / Rolls, Melissa; Webster, Paul; Balba, Nader H.; Rose, John K.

In: Cell, Vol. 79, No. 3, 04.11.1994, p. 497-506.

Research output: Contribution to journalArticle

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