Novel multiplex single nucleotide polymorphism-based method for identifying epidemic clones of listeria monocytogenes

Sara Lomonaco, Stephen J. Knabel, Alessandra Dalmasso, Tiziana Civera, Maria Teresa Bottero

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A novel primer extension-based, multiplex minisequencing assay targeting six highly informative single nucleotide polymorphisms (SNPs) in four virulence genes correctly identified and differentiated all four epidemic clones (ECs) of Listeria monocytogenes and 9 other strains initially misclassified as non-ECs. This assay allows rapid, accurate, and high-throughput screening for all known ECs of L. monocytogenes.

Original languageEnglish (US)
Pages (from-to)6290-6294
Number of pages5
JournalApplied and environmental microbiology
Volume77
Issue number17
DOIs
StatePublished - Sep 1 2011

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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