A nested polymerase chain reaction (PCR) protocol using unique primers was developed to detect and quantify Myxococcus species from environmental samples. The protocol amplified most species of Myxococcus when 10 pg of DNA representing 1000 cells was present, although over half were amplified with as little as 0.1 pg (10 cells). The protocol did not amplify other myxobacterial species, members of the δ-proteobacteria or other unrelated organisms tested at significantly higher concentrations of DNA. The primers were also used in quantitative PCRs, which accurately estimated the population levels in soil.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)