Novel primers for detection and quantification of Myxococcus species in situ

K. J. Martin, Carolee Theresa Bull

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A nested polymerase chain reaction (PCR) protocol using unique primers was developed to detect and quantify Myxococcus species from environmental samples. The protocol amplified most species of Myxococcus when 10 pg of DNA representing 1000 cells was present, although over half were amplified with as little as 0.1 pg (10 cells). The protocol did not amplify other myxobacterial species, members of the δ-proteobacteria or other unrelated organisms tested at significantly higher concentrations of DNA. The primers were also used in quantitative PCRs, which accurately estimated the population levels in soil.

Original languageEnglish (US)
Pages (from-to)773-775
Number of pages3
JournalMolecular Ecology Notes
Volume6
Issue number3
DOIs
StatePublished - Sep 1 2006

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Myxococcus
Proteobacteria
Polymerase Chain Reaction
Polymerase chain reaction
DNA
quantitative polymerase chain reaction
Soil
polymerase chain reaction
cells
Soils
organisms
Population
soil
sampling
detection
protocol
in situ

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Ecology

Cite this

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Novel primers for detection and quantification of Myxococcus species in situ. / Martin, K. J.; Bull, Carolee Theresa.

In: Molecular Ecology Notes, Vol. 6, No. 3, 01.09.2006, p. 773-775.

Research output: Contribution to journalArticle

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