Novel splice variant of mouse insulin2 mRNA: Implications for insulin expression

Amaresh C. Panda, Shardul D. Kulkarni, Bhavana Muralidharan, Baskar Bakthavachalu, Vasudevan Seshadri

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Insulin is a secreted peptide that controls glucose homeostasis in mammals, and insulin biosynthesis is regulated by glucose at many levels. Rodent insulin is encoded by two non-allelic genes. We have identified a novel splice variant of the insulin2 gene in mice that constitutes about 75% of total insulin2 mRNA. The alternate splicing does not alter the ORF but reduces the 5′UTR by 12 bases. A reporter gene containing the novel short 5′UTR, is more efficiently expressed in cells, suggesting that alternative splicing of insulin mRNA in mice could result in an additional level of regulation in insulin biosynthesis.

Original languageEnglish (US)
Pages (from-to)1169-1173
Number of pages5
JournalFEBS Letters
Volume584
Issue number6
DOIs
StatePublished - Mar 2010

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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