Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses

Kasumi Inokuchi, Fumiaki Imamura, Haruki Takeuchi, Ryang Kim, Hiroyuki Okuno, Hirofumi Nishizumi, Haruhiko Bito, Takefumi Kikusui, Hitoshi Sakano

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Odour information induces various innate responses that are critical to the survival of the individual and for the species. An axon guidance molecule, Neuropilin 2 (Nrp2), is known to mediate targeting of olfactory sensory neurons (primary neurons), to the posteroventral main olfactory bulb (PV MOB) in mice. Here we report that Nrp2-positive (Nrp2+) mitral cells (MCs, second-order neurons) play crucial roles in transmitting attractive social signals from the PV MOB to the anterior part of medial amygdala (MeA). Semaphorin 3F, a repulsive ligand to Nrp2, regulates both migration of Nrp2+ MCs to the PV MOB and their axonal projection to the anterior MeA. In the MC-specific Nrp2 knockout mice, circuit formation of Nrp2+ MCs and odour-induced attractive social responses are impaired. In utero, electroporation demonstrates that activation of the Nrp2 gene in MCs is sufficient to instruct their circuit formation from the PV MOB to the anterior MeA.

Original languageEnglish (US)
Number of pages1
JournalNature communications
Volume8
DOIs
StatePublished - Jul 21 2017

Fingerprint

Neuropilin-2
odors
bulbs
Odors
neurons
Networks (circuits)
Olfactory Bulb
cells
knockout mice
Amygdala
Neurons
axons
genes
mice
projection
Semaphorins
Olfactory Receptor Neurons
activation
ligands
Electroporation

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Inokuchi, Kasumi ; Imamura, Fumiaki ; Takeuchi, Haruki ; Kim, Ryang ; Okuno, Hiroyuki ; Nishizumi, Hirofumi ; Bito, Haruhiko ; Kikusui, Takefumi ; Sakano, Hitoshi. / Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses. In: Nature communications. 2017 ; Vol. 8.
@article{67953f7154894b0d8cc1f4395b1f777d,
title = "Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses",
abstract = "Odour information induces various innate responses that are critical to the survival of the individual and for the species. An axon guidance molecule, Neuropilin 2 (Nrp2), is known to mediate targeting of olfactory sensory neurons (primary neurons), to the posteroventral main olfactory bulb (PV MOB) in mice. Here we report that Nrp2-positive (Nrp2+) mitral cells (MCs, second-order neurons) play crucial roles in transmitting attractive social signals from the PV MOB to the anterior part of medial amygdala (MeA). Semaphorin 3F, a repulsive ligand to Nrp2, regulates both migration of Nrp2+ MCs to the PV MOB and their axonal projection to the anterior MeA. In the MC-specific Nrp2 knockout mice, circuit formation of Nrp2+ MCs and odour-induced attractive social responses are impaired. In utero, electroporation demonstrates that activation of the Nrp2 gene in MCs is sufficient to instruct their circuit formation from the PV MOB to the anterior MeA.",
author = "Kasumi Inokuchi and Fumiaki Imamura and Haruki Takeuchi and Ryang Kim and Hiroyuki Okuno and Hirofumi Nishizumi and Haruhiko Bito and Takefumi Kikusui and Hitoshi Sakano",
year = "2017",
month = "7",
day = "21",
doi = "10.1038/ncomms15977",
language = "English (US)",
volume = "8",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses. / Inokuchi, Kasumi; Imamura, Fumiaki; Takeuchi, Haruki; Kim, Ryang; Okuno, Hiroyuki; Nishizumi, Hirofumi; Bito, Haruhiko; Kikusui, Takefumi; Sakano, Hitoshi.

In: Nature communications, Vol. 8, 21.07.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses

AU - Inokuchi, Kasumi

AU - Imamura, Fumiaki

AU - Takeuchi, Haruki

AU - Kim, Ryang

AU - Okuno, Hiroyuki

AU - Nishizumi, Hirofumi

AU - Bito, Haruhiko

AU - Kikusui, Takefumi

AU - Sakano, Hitoshi

PY - 2017/7/21

Y1 - 2017/7/21

N2 - Odour information induces various innate responses that are critical to the survival of the individual and for the species. An axon guidance molecule, Neuropilin 2 (Nrp2), is known to mediate targeting of olfactory sensory neurons (primary neurons), to the posteroventral main olfactory bulb (PV MOB) in mice. Here we report that Nrp2-positive (Nrp2+) mitral cells (MCs, second-order neurons) play crucial roles in transmitting attractive social signals from the PV MOB to the anterior part of medial amygdala (MeA). Semaphorin 3F, a repulsive ligand to Nrp2, regulates both migration of Nrp2+ MCs to the PV MOB and their axonal projection to the anterior MeA. In the MC-specific Nrp2 knockout mice, circuit formation of Nrp2+ MCs and odour-induced attractive social responses are impaired. In utero, electroporation demonstrates that activation of the Nrp2 gene in MCs is sufficient to instruct their circuit formation from the PV MOB to the anterior MeA.

AB - Odour information induces various innate responses that are critical to the survival of the individual and for the species. An axon guidance molecule, Neuropilin 2 (Nrp2), is known to mediate targeting of olfactory sensory neurons (primary neurons), to the posteroventral main olfactory bulb (PV MOB) in mice. Here we report that Nrp2-positive (Nrp2+) mitral cells (MCs, second-order neurons) play crucial roles in transmitting attractive social signals from the PV MOB to the anterior part of medial amygdala (MeA). Semaphorin 3F, a repulsive ligand to Nrp2, regulates both migration of Nrp2+ MCs to the PV MOB and their axonal projection to the anterior MeA. In the MC-specific Nrp2 knockout mice, circuit formation of Nrp2+ MCs and odour-induced attractive social responses are impaired. In utero, electroporation demonstrates that activation of the Nrp2 gene in MCs is sufficient to instruct their circuit formation from the PV MOB to the anterior MeA.

UR - http://www.scopus.com/inward/record.url?scp=85043776825&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85043776825&partnerID=8YFLogxK

U2 - 10.1038/ncomms15977

DO - 10.1038/ncomms15977

M3 - Article

C2 - 28731029

AN - SCOPUS:85043776825

VL - 8

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

ER -