NUC-2, a component of the phosphate-regulated signal transduction pathway in Neurospora crassa, is an ankyrin repeat protein

Yoav Peleg, Rodolfo Aramayo, Seogchan Kang, Jeff G. Hall, Robert L. Metzenberg

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In response to phosphorus limitation, the fungus Neurospora crassa synthesizes a number of enzymes that function to bring more phosphate into the cell. The NUC-2 protein appears to sense the availability of phosphate and transmits the signal downstream to the regulatory pathway. The nuc-2+ gene has been cloned by its ability to restore growth of a nuc-2 mutant under restrictive conditions of high pH and low phosphate concentration. We mapped the cloned gene to the right arm of linkage group II, consistent with the chromosomal position of the nuc-2 mutation as determined by classical genetic mapping. The nuc-2+ open reading frame is interrupted by five introns and codes for a protein of 1066 amino acid residues. Its predicted amino acid sequence has high similarity to that of its homolog in Saccharomyces cerevisiae, PHO81. Both proteins contain six ankyrin repeats, which have been implicated in the cyclin-dependent kinase inhibitory activity of PHO81. The phenotypes of a nuc-2 mutant generated by repeat-induced point mutation and of a strain harboring a UV-induced nuc-2 allele are indistinguishable. Both are unable to grow under the restrictive conditions, a phenotype which is to some degree temperature dependent. The nuc-2+ gene is transcriptionally regulated. A 15-fold increase in the level of the nuc-2+ transcript occurs in response to a decrease in exogenous phosphate concentration.

Original languageEnglish (US)
Pages (from-to)709-716
Number of pages8
JournalMolecular and General Genetics
Volume252
Issue number6
DOIs
StatePublished - Nov 21 1996

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Ankyrin Repeat
Neurospora crassa
Signal Transduction
Phosphates
Proteins
Genes
Phenotype
Cyclin-Dependent Kinases
Point Mutation
Introns
Phosphorus
Open Reading Frames
Saccharomyces cerevisiae
Amino Acid Sequence
Fungi
Alleles
Amino Acids
Mutation
Temperature
Enzymes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Peleg, Yoav ; Aramayo, Rodolfo ; Kang, Seogchan ; Hall, Jeff G. ; Metzenberg, Robert L. / NUC-2, a component of the phosphate-regulated signal transduction pathway in Neurospora crassa, is an ankyrin repeat protein. In: Molecular and General Genetics. 1996 ; Vol. 252, No. 6. pp. 709-716.
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NUC-2, a component of the phosphate-regulated signal transduction pathway in Neurospora crassa, is an ankyrin repeat protein. / Peleg, Yoav; Aramayo, Rodolfo; Kang, Seogchan; Hall, Jeff G.; Metzenberg, Robert L.

In: Molecular and General Genetics, Vol. 252, No. 6, 21.11.1996, p. 709-716.

Research output: Contribution to journalArticle

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