33 Citations (Scopus)

Abstract

Treatment of rats with low doses of hepatocarcinogens is associated with a number of phenomena, including nuclear enlargement and altered nucleocytoplasmic compartmentation, which potentially reflect initiatory changes. Since a number of investigations have indicated that changes in ploidy may relate to the development of altered foci and/or hepatocellular carcinomas in liver, and since nuclear enlargement may be associated with changes in DNA content, we examined the ploidy of rat hepatocytes following essentially nontoxic low-dose carcinogen treatment. Treatments with thioacetamide, 2-acetylaminofluorene, or dimethylnitrosamine were all associated with a notable shift of nuclei from diploid to tetraploid, in the apparent absence of nuclear replication. These changes were similar in magnitude to that associated with the substantial liver regeneration induced by carbon tetrachloride. If it is argued that cell replication is a necessary prerequisite for the completion of initiation, these data suggest that there may be thresholds for initiation.

Original languageEnglish (US)
Pages (from-to)1304-1308
Number of pages5
JournalCancer Research
Volume52
Issue number5
StatePublished - Mar 1992

Fingerprint

Ploidies
Thioacetamide
2-Acetylaminofluorene
Dimethylnitrosamine
Liver Regeneration
Tetraploidy
Carbon Tetrachloride
Diploidy
Carcinogens
Hepatocytes
Hepatocellular Carcinoma
Liver
DNA

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Clawson, G. A., Rhame, J. G., & Wilkinson, D. S. (1992). Nuclear Enlargement Induced by Hepatocarcinogens Alters Ploidy. Cancer Research, 52(5), 1304-1308.
Clawson, Gary A. ; Rhame, Jean G. ; Wilkinson, David S. / Nuclear Enlargement Induced by Hepatocarcinogens Alters Ploidy. In: Cancer Research. 1992 ; Vol. 52, No. 5. pp. 1304-1308.
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title = "Nuclear Enlargement Induced by Hepatocarcinogens Alters Ploidy",
abstract = "Treatment of rats with low doses of hepatocarcinogens is associated with a number of phenomena, including nuclear enlargement and altered nucleocytoplasmic compartmentation, which potentially reflect initiatory changes. Since a number of investigations have indicated that changes in ploidy may relate to the development of altered foci and/or hepatocellular carcinomas in liver, and since nuclear enlargement may be associated with changes in DNA content, we examined the ploidy of rat hepatocytes following essentially nontoxic low-dose carcinogen treatment. Treatments with thioacetamide, 2-acetylaminofluorene, or dimethylnitrosamine were all associated with a notable shift of nuclei from diploid to tetraploid, in the apparent absence of nuclear replication. These changes were similar in magnitude to that associated with the substantial liver regeneration induced by carbon tetrachloride. If it is argued that cell replication is a necessary prerequisite for the completion of initiation, these data suggest that there may be thresholds for initiation.",
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Clawson, GA, Rhame, JG & Wilkinson, DS 1992, 'Nuclear Enlargement Induced by Hepatocarcinogens Alters Ploidy', Cancer Research, vol. 52, no. 5, pp. 1304-1308.

Nuclear Enlargement Induced by Hepatocarcinogens Alters Ploidy. / Clawson, Gary A.; Rhame, Jean G.; Wilkinson, David S.

In: Cancer Research, Vol. 52, No. 5, 03.1992, p. 1304-1308.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nuclear Enlargement Induced by Hepatocarcinogens Alters Ploidy

AU - Clawson, Gary A.

AU - Rhame, Jean G.

AU - Wilkinson, David S.

PY - 1992/3

Y1 - 1992/3

N2 - Treatment of rats with low doses of hepatocarcinogens is associated with a number of phenomena, including nuclear enlargement and altered nucleocytoplasmic compartmentation, which potentially reflect initiatory changes. Since a number of investigations have indicated that changes in ploidy may relate to the development of altered foci and/or hepatocellular carcinomas in liver, and since nuclear enlargement may be associated with changes in DNA content, we examined the ploidy of rat hepatocytes following essentially nontoxic low-dose carcinogen treatment. Treatments with thioacetamide, 2-acetylaminofluorene, or dimethylnitrosamine were all associated with a notable shift of nuclei from diploid to tetraploid, in the apparent absence of nuclear replication. These changes were similar in magnitude to that associated with the substantial liver regeneration induced by carbon tetrachloride. If it is argued that cell replication is a necessary prerequisite for the completion of initiation, these data suggest that there may be thresholds for initiation.

AB - Treatment of rats with low doses of hepatocarcinogens is associated with a number of phenomena, including nuclear enlargement and altered nucleocytoplasmic compartmentation, which potentially reflect initiatory changes. Since a number of investigations have indicated that changes in ploidy may relate to the development of altered foci and/or hepatocellular carcinomas in liver, and since nuclear enlargement may be associated with changes in DNA content, we examined the ploidy of rat hepatocytes following essentially nontoxic low-dose carcinogen treatment. Treatments with thioacetamide, 2-acetylaminofluorene, or dimethylnitrosamine were all associated with a notable shift of nuclei from diploid to tetraploid, in the apparent absence of nuclear replication. These changes were similar in magnitude to that associated with the substantial liver regeneration induced by carbon tetrachloride. If it is argued that cell replication is a necessary prerequisite for the completion of initiation, these data suggest that there may be thresholds for initiation.

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