Nuclear proteomic changes linked to soybean rust resistance

Bret Cooper, Kimberly B. Campbell, Jian Feng, Wesley M. Garrett, Reid Frederick

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Soybean rust, caused by the fungus Phakopsora pachyrhizi, is an emerging threat to the US soybean crop. In an effort to identify proteins that contribute to disease resistance in soybean we compared a susceptible Williams 82 cultivar to a resistant Williams 82 inbred isoline harboring the Rpp1 resistance gene (R-gene). Approximately 4975 proteins from nuclear preparations of leaves were detected using a high-throughput liquid chromatography-mass spectrometry method. Many of these proteins have predicted nuclear localization signals, have homology to transcription factors and other nuclear regulatory proteins, and are phosphorylated. Statistics of summed spectral counts revealed sets of proteins with differential accumulation changes between susceptible and resistant plants. These protein accumulation changes were compared to previously reported gene expression changes and very little overlap was found. Thus, it appears that numerous proteins are post-translationally affected in the nucleus after infection. To our knowledge, this is the first indication of large-scale proteomic change in a plant nucleus after infection. Furthermore, the data reveal distinct proteins under control of Rpp1 and show that this disease resistance gene regulates nuclear protein accumulation. These regulated proteins likely influence broader defense responses, and these data may facilitate the development of plants with improved resistance.

Original languageEnglish (US)
Pages (from-to)773-783
Number of pages11
JournalMolecular BioSystems
Volume7
Issue number3
DOIs
StatePublished - Mar 1 2011

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Soybeans
Proteomics
Nuclear Proteins
Proteins
Disease Resistance
Nuclear Localization Signals
Plant Development
Infection
Liquid Chromatography
Genes
Mass Spectrometry
Fungi
Transcription Factors
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology

Cite this

Cooper, B., Campbell, K. B., Feng, J., Garrett, W. M., & Frederick, R. (2011). Nuclear proteomic changes linked to soybean rust resistance. Molecular BioSystems, 7(3), 773-783. https://doi.org/10.1039/c0mb00171f
Cooper, Bret ; Campbell, Kimberly B. ; Feng, Jian ; Garrett, Wesley M. ; Frederick, Reid. / Nuclear proteomic changes linked to soybean rust resistance. In: Molecular BioSystems. 2011 ; Vol. 7, No. 3. pp. 773-783.
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Cooper, B, Campbell, KB, Feng, J, Garrett, WM & Frederick, R 2011, 'Nuclear proteomic changes linked to soybean rust resistance', Molecular BioSystems, vol. 7, no. 3, pp. 773-783. https://doi.org/10.1039/c0mb00171f

Nuclear proteomic changes linked to soybean rust resistance. / Cooper, Bret; Campbell, Kimberly B.; Feng, Jian; Garrett, Wesley M.; Frederick, Reid.

In: Molecular BioSystems, Vol. 7, No. 3, 01.03.2011, p. 773-783.

Research output: Contribution to journalArticle

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Cooper B, Campbell KB, Feng J, Garrett WM, Frederick R. Nuclear proteomic changes linked to soybean rust resistance. Molecular BioSystems. 2011 Mar 1;7(3):773-783. https://doi.org/10.1039/c0mb00171f