Nuclear Stiffening Inhibits Migration of Invasive Melanoma Cells

Alexandre J.S. Ribeiro, Payal Khanna, Aishwarya Sukumar, Cheng Dong, Kris Noel Dahl

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

During metastasis, melanoma cells must be sufficiently deformable to squeeze through extracellular barriers with small pore sizes. We visualize and quantify deformability of single cells using micropipette aspiration and examine the migration potential of a population of melanoma cells using a flow migration apparatus. We artificially stiffen the nucleus with recombinant overexpression of Δ50 lamin A, which is found in patients with Hutchison Gilford progeria syndrome and in aged individuals. Melanoma cells, both WM35 and Lu1205, both show reduced nuclear deformability and reduced cell invasion with the expression of Δ50 lamin A. These studies suggest that cellular aging including expression of Δ50 lamin A and nuclear stiffening may reduce the potential for metastatic cancer migration. Thus, the pathway of cancer metastasis may be kept in check by mechanical factors in addition to known chemical pathway regulation.

Original languageEnglish (US)
Pages (from-to)544-551
Number of pages8
JournalCellular and Molecular Bioengineering
Volume7
Issue number4
DOIs
StatePublished - Jan 1 2014

Fingerprint

Lamin Type A
Melanoma
Formability
Migration
Cell
Metastasis
Pore size
Aging of materials
Pathway
Cancer
Progeria
Neoplasm Metastasis
Cell Aging
Invasion
Nucleus
Neoplasms
Quantify
Population

All Science Journal Classification (ASJC) codes

  • Modeling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ribeiro, Alexandre J.S. ; Khanna, Payal ; Sukumar, Aishwarya ; Dong, Cheng ; Dahl, Kris Noel. / Nuclear Stiffening Inhibits Migration of Invasive Melanoma Cells. In: Cellular and Molecular Bioengineering. 2014 ; Vol. 7, No. 4. pp. 544-551.
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Nuclear Stiffening Inhibits Migration of Invasive Melanoma Cells. / Ribeiro, Alexandre J.S.; Khanna, Payal; Sukumar, Aishwarya; Dong, Cheng; Dahl, Kris Noel.

In: Cellular and Molecular Bioengineering, Vol. 7, No. 4, 01.01.2014, p. 544-551.

Research output: Contribution to journalArticle

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AB - During metastasis, melanoma cells must be sufficiently deformable to squeeze through extracellular barriers with small pore sizes. We visualize and quantify deformability of single cells using micropipette aspiration and examine the migration potential of a population of melanoma cells using a flow migration apparatus. We artificially stiffen the nucleus with recombinant overexpression of Δ50 lamin A, which is found in patients with Hutchison Gilford progeria syndrome and in aged individuals. Melanoma cells, both WM35 and Lu1205, both show reduced nuclear deformability and reduced cell invasion with the expression of Δ50 lamin A. These studies suggest that cellular aging including expression of Δ50 lamin A and nuclear stiffening may reduce the potential for metastatic cancer migration. Thus, the pathway of cancer metastasis may be kept in check by mechanical factors in addition to known chemical pathway regulation.

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