Nucleic acid chromatography: Substitute solid support material for RPC-5 columns

J. M. Flanagan, R. K. Fujimura, K. Bruce Jacobson

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Chromatography of tRNA and DNA fragments on columns of reverse-phase 5 (RPC-5) exchange material has been widely employed for analytical and preparative studies. The Plaskon bead that formed the solid support on which a quaternary amine was absorbed is no longer commercially available. A Voltalef bead is available and provides similar, though not identical, chromatograms for Asp-tRNA, Ser-tRNA, and certain DNA fragments. Procedures are described for preparation of the column packing and for long-term operation of the column.

Original languageEnglish (US)
Pages (from-to)299-304
Number of pages6
JournalAnalytical Biochemistry
Volume153
Issue number2
DOIs
StatePublished - Mar 1986

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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