Nucleosome-like, single-stranded DNA (ssDNA)-histone octamer complexes and the implication for DNA double strand break repair

Nicholas L. Adkins, Sarah G. Swygert, Parminder Kaur, Hengyao Niu, Sergei A. Grigoryev, Patrick Sung, Hong Wang, Craig L. Peterson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Repair of DNA double strand breaks (DSBs) is key for maintenance of genome integrity. When DSBs are repaired by homologous recombination, DNA ends can undergo extensive processing, producing long stretches of single-stranded DNA (ssDNA). In vivo, DSB processing occurs in the context of chromatin, and studies indicate that histones may remain associated with processed DSBs. Here we demonstrate that histones are not evicted from ssDNA after in vitro chromatin resection. In addition, we reconstitute histone-ssDNA complexes (termed ssNucs) with ssDNA and recombinant histones and analyze these particles by a combination of native gel electrophoresis, sedimentation velocity, electron microscopy, and a recently developed electrostatic force microscopy technique, DREEM (dual-resonance frequency-enhanced electrostatic force microscopy). The reconstituted ssNucs are homogenous and relatively stable, and DREEM reveals ssDNA wrapping around histones. We also find that histone octamers are easily transferred in trans from ssNucs to either double-stranded DNA or ssDNA. Furthermore, the Fun30 remodeling enzyme, which has been implicated in DNA repair, binds ssNucs preferentially over nucleosomes, and ssNucs are effective at activating Fun30 ATPase activity. Our results indicate that ssNucs may be a hallmark of processes that generate ssDNA, and that posttranslational modification of ssNucs may generate novel signaling platforms involved in genome stability.

Original languageEnglish (US)
Pages (from-to)5271-5281
Number of pages11
JournalJournal of Biological Chemistry
Volume292
Issue number13
DOIs
StatePublished - Mar 31 2017

Fingerprint

Double-Stranded DNA Breaks
Nucleosomes
Single-Stranded DNA
Histones
Repair
DNA
Electrostatic force
Atomic Force Microscopy
Static Electricity
Microscopic examination
Chromatin
Genes
Genomic Instability
Homologous Recombination
Post Translational Protein Processing
Processing
Electrophoresis
Sedimentation
DNA Repair
Electron microscopy

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Adkins, Nicholas L. ; Swygert, Sarah G. ; Kaur, Parminder ; Niu, Hengyao ; Grigoryev, Sergei A. ; Sung, Patrick ; Wang, Hong ; Peterson, Craig L. / Nucleosome-like, single-stranded DNA (ssDNA)-histone octamer complexes and the implication for DNA double strand break repair. In: Journal of Biological Chemistry. 2017 ; Vol. 292, No. 13. pp. 5271-5281.
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Nucleosome-like, single-stranded DNA (ssDNA)-histone octamer complexes and the implication for DNA double strand break repair. / Adkins, Nicholas L.; Swygert, Sarah G.; Kaur, Parminder; Niu, Hengyao; Grigoryev, Sergei A.; Sung, Patrick; Wang, Hong; Peterson, Craig L.

In: Journal of Biological Chemistry, Vol. 292, No. 13, 31.03.2017, p. 5271-5281.

Research output: Contribution to journalArticle

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T1 - Nucleosome-like, single-stranded DNA (ssDNA)-histone octamer complexes and the implication for DNA double strand break repair

AU - Adkins, Nicholas L.

AU - Swygert, Sarah G.

AU - Kaur, Parminder

AU - Niu, Hengyao

AU - Grigoryev, Sergei A.

AU - Sung, Patrick

AU - Wang, Hong

AU - Peterson, Craig L.

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