Nucleosome organization in the Drosophila genome

Travis N. Mavrich, Cizhong Jiang, Ilya P. Ioshikhes, Xiaoyong Li, Bryan J. Venters, Sara J. Zanton, Lynn P. Tomsho, Ji Qi, Robert L. Glaser, Stephan C. Schuster, David S. Gilmour, Istvan Albert, B. Franklin Pugh

Research output: Contribution to journalArticlepeer-review

532 Scopus citations

Abstract

Comparative genomics of nucleosome positions provides a powerful means for understanding how the organization of chromatin and the transcription machinery co-evolve. Here we produce a high-resolution reference map of H2A.Z and bulk nucleosome locations across the genome of the fly Drosophila melanogaster and compare it to that from the yeast Saccharomyces cerevisiae. Like Saccharomyces, Drosophila nucleosomes are organized around active transcription start sites in a canonical -1, nucleosome-free region, +1 arrangement. However, Drosophila does not incorporate H2A.Z into the -1 nucleosome and does not bury its transcriptional start site in the +1 nucleosome. At thousands of genes, RNA polymerase II engages the +1 nucleosome and pauses. How the transcription initiation machinery contends with the +1 nucleosome seems to be fundamentally different across major eukaryotic lines.

Original languageEnglish (US)
Pages (from-to)358-362
Number of pages5
JournalNature
Volume453
Issue number7193
DOIs
StatePublished - May 15 2008

All Science Journal Classification (ASJC) codes

  • General

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