Nucleosome structure and function

Robert K. McGinty, Song Tan

Research output: Contribution to journalReview article

107 Citations (Scopus)

Abstract

Major studies have been made in the understanding of nucleosome structure and function over the years. Co-crystallization and comprehensive NMR analysis of chromatin factors and the nucleosome core particle have established the acidic patch arginine-anchor as a paradigm for nucleosome recognition. Advancements in cryo-EM technology have aided in the visualization of the two-start 30 nm fiber and characterization of nucleosome binding by large and conformationally flexible chromatin enzymes. More recently, Tosi and colleagues used cryo-EM and cross-linking mass spectrometry (XL-MS) to extensively characterize the interaction of the holo-INO80 complex with the nucleosome core particle (NCP). NCP bound cryo-EM reconstitutions have also been reported for the Saccharomyces cerevisiae Piccolo NuA4 histone acetyltransferase (HAT) complex and the HP1-like heterochromatin protein Swi6 from Schizosaccharomyces pombe.

Original languageEnglish (US)
Pages (from-to)2255-2273
Number of pages19
JournalChemical Reviews
Volume115
Issue number6
DOIs
StatePublished - Mar 25 2015

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Nucleosomes
Chromatin
Histone Acetyltransferases
Crystallization
Anchors
Yeast
Mass spectrometry
Arginine
Visualization
Nuclear magnetic resonance
Fibers
Enzymes
Proteins

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

McGinty, Robert K. ; Tan, Song. / Nucleosome structure and function. In: Chemical Reviews. 2015 ; Vol. 115, No. 6. pp. 2255-2273.
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Nucleosome structure and function. / McGinty, Robert K.; Tan, Song.

In: Chemical Reviews, Vol. 115, No. 6, 25.03.2015, p. 2255-2273.

Research output: Contribution to journalReview article

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