Nucleotide degradation and ribose salvage in yeast

Yi Fan Xu, Fabien Létisse, Farnaz Absalan, Wenyun Lu, Ekaterina Kuznetsova, Greg Brown, Amy A. Caudy, Alexander F. Yakunin, James R. Broach, Joshua D. Rabinowitz

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Nucleotide degradation is a universal metabolic capability. Here we combine metabolomics, genetics and biochemistry to characterize the yeast pathway. Nutrient starvation, via PKA, AMPK/SNF1, and TOR, triggers autophagic breakdown of ribosomes into nucleotides. A protein not previously associated with nucleotide degradation, Phm8, converts nucleotide monophosphates into nucleosides. Downstream steps, which involve the purine nucleoside phosphorylase, Pnp1, and pyrimidine nucleoside hydrolase, Urh1, funnel ribose into the nonoxidative pentose phosphate pathway. During carbon starvation, the ribose-derived carbon accumulates as sedoheptulose-7-phosphate, whose consumption by transaldolase is impaired due to depletion of transaldolase's other substrate, glyceraldehyde-3-phosphate. Oxidative stress increases glyceraldehyde-3-phosphate, resulting in rapid consumption of sedoheptulose-7-phosphate to make NADPH for antioxidant defense. Ablation of Phm8 or double deletion of Pnp1 and Urh1 prevent effective nucleotide salvage, resulting in metabolite depletion and impaired survival of starving yeast. Thus, ribose salvage provides means of surviving nutrient starvation and oxidative stress.

Original languageEnglish (US)
Article number665
JournalMolecular Systems Biology
Volume9
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Salvaging
Ribose
ribose
Nucleotides
Phosphate
Yeast
Phosphates
Degradation
Yeasts
nucleotides
yeasts
transaldolase
Transaldolase
Starvation
glyceraldehyde 3-phosphate
Glyceraldehyde 3-Phosphate
degradation
Oxidative Stress
starvation
Oxidative stress

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)
  • Applied Mathematics

Cite this

Xu, Y. F., Létisse, F., Absalan, F., Lu, W., Kuznetsova, E., Brown, G., ... Rabinowitz, J. D. (2013). Nucleotide degradation and ribose salvage in yeast. Molecular Systems Biology, 9(1), [665]. https://doi.org/10.1038/msb.2013.21
Xu, Yi Fan ; Létisse, Fabien ; Absalan, Farnaz ; Lu, Wenyun ; Kuznetsova, Ekaterina ; Brown, Greg ; Caudy, Amy A. ; Yakunin, Alexander F. ; Broach, James R. ; Rabinowitz, Joshua D. / Nucleotide degradation and ribose salvage in yeast. In: Molecular Systems Biology. 2013 ; Vol. 9, No. 1.
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Xu, YF, Létisse, F, Absalan, F, Lu, W, Kuznetsova, E, Brown, G, Caudy, AA, Yakunin, AF, Broach, JR & Rabinowitz, JD 2013, 'Nucleotide degradation and ribose salvage in yeast', Molecular Systems Biology, vol. 9, no. 1, 665. https://doi.org/10.1038/msb.2013.21

Nucleotide degradation and ribose salvage in yeast. / Xu, Yi Fan; Létisse, Fabien; Absalan, Farnaz; Lu, Wenyun; Kuznetsova, Ekaterina; Brown, Greg; Caudy, Amy A.; Yakunin, Alexander F.; Broach, James R.; Rabinowitz, Joshua D.

In: Molecular Systems Biology, Vol. 9, No. 1, 665, 01.01.2013.

Research output: Contribution to journalArticle

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AU - Caudy, Amy A.

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AU - Rabinowitz, Joshua D.

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Xu YF, Létisse F, Absalan F, Lu W, Kuznetsova E, Brown G et al. Nucleotide degradation and ribose salvage in yeast. Molecular Systems Biology. 2013 Jan 1;9(1). 665. https://doi.org/10.1038/msb.2013.21