Nucleus of Circulating Tumor Cell Determines Its Translocation Through Biomimetic Microconstrictions and Its Physical Enrichment by Microfiltration

Yiqiu Xia, Yuan Wan, Sijie Hao, Merisa Nisic, Ramdane A. Harouaka, Yizhu Chen, Xin Zou, Siyang Zheng

Research output: Contribution to journalArticle

Abstract

The mechanism of cells passing through microconstrictions, such as capillaries and endothelial junctions, influences metastasis of circulating tumor cells (CTCs) in vivo, as well as size-based enrichment of CTCs in vitro. However, very few studies observe such translocation of microconstrictions in real time, and thus the inherent biophysical mechanism is poorly understood. In this study, a multiplexed microfluidic device is fabricated for real-time tracking of cell translocation under physiological pressure and recording deformation of the whole cell and nucleus, respectively. It is found that the deformability and size of the nucleus instead of the whole cell dominate cellular translocation through microconstrictions under a normal physiological pressure range. More specifically, cells with a large and stiff nucleus are prone to be blocked by relatively small constrictions. The same phenomenon is also observed in the size-based enrichment of CTCs from peripheral blood of metastatic cancer patients. These findings are different from a popular viewpoint that the size and deformability of a whole cell mainly determine cell translation through microconstrictions, and thus may elucidate interactions between CTCs and capillaries from a new perspective and guide the rational design of size-based microfilters for rare cell enrichment.

Original languageEnglish (US)
Article number1802899
JournalSmall
Volume14
Issue number44
DOIs
StatePublished - Nov 2 2018

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Circulating Neoplastic Cells
Biomimetics
Microfiltration
Tumors
Cells
Formability
Lab-On-A-Chip Devices
Microfluidics
Cell Tracking
Pressure
Blood
Cell Nucleus
Constriction
Neoplasm Metastasis
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

Cite this

Xia, Yiqiu ; Wan, Yuan ; Hao, Sijie ; Nisic, Merisa ; Harouaka, Ramdane A. ; Chen, Yizhu ; Zou, Xin ; Zheng, Siyang. / Nucleus of Circulating Tumor Cell Determines Its Translocation Through Biomimetic Microconstrictions and Its Physical Enrichment by Microfiltration. In: Small. 2018 ; Vol. 14, No. 44.
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Nucleus of Circulating Tumor Cell Determines Its Translocation Through Biomimetic Microconstrictions and Its Physical Enrichment by Microfiltration. / Xia, Yiqiu; Wan, Yuan; Hao, Sijie; Nisic, Merisa; Harouaka, Ramdane A.; Chen, Yizhu; Zou, Xin; Zheng, Siyang.

In: Small, Vol. 14, No. 44, 1802899, 02.11.2018.

Research output: Contribution to journalArticle

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AU - Xia, Yiqiu

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AU - Nisic, Merisa

AU - Harouaka, Ramdane A.

AU - Chen, Yizhu

AU - Zou, Xin

AU - Zheng, Siyang

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