O6-alkylguanine-DNA alkyltransferase activity in normal human tissues and cells

Curtis C. Harris, Anthony Pegg, Roland C. Grafstrom, Benjamin F. Trump

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Normal adult human tissues and cultured bronchial epithelial cells and fibroblasts exhibit 06-alkylguanine-DNA alkyltransferase activity in vitro by catalyzing the repair of the promutagenic alkylation lesion 06-methylguanine from DNA. The amount repaired by extracts of liver, peripheral lung, and colon extracts was proportional to the amount of extract protein. Repair of O6-methylguanine led to stoichiometric regeneration of guanine in the DNA and stoichiometric formation of S-methylcysteine in protein. Alkyltransferase activity varies in the different human tissues tested in the decreasing order of liver > colon > esophagus > peripheral lung > brain. Extracts of lung tissues, cultured human bronchial epithelial cells, and fibroblasts had similar alkyltransferase activities. Various human tissues exhibit 2- to 10fold higher alkyltransferase activity than corresponding rat tissues. Whereas the interindividual variation of the activity was 4-to 5-fold in ten or more human lung and colon specimens, the interindividual variation in the inbred rat was less than 20%. The present results show that different human tissues and cells have a several-fold higher capacity to repair 06-methyiguanine in DNA than do rat tissues and that the repair process occurs via a mechanism similar to that shown previously in other mammalian cells and Escherichia coli.

Original languageEnglish (US)
Pages (from-to)2855-2857
Number of pages3
JournalCancer Research
Volume44
Issue number7
StatePublished - Jul 1 1984

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Alkyl and Aryl Transferases
Lung
Colon
DNA
Fibroblasts
Epithelial Cells
Liver Extracts
Tissue Extracts
Guanine
Alkylation
Esophagus
DNA alkyltransferase
Regeneration
Proteins
Escherichia coli
Liver
Brain

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Harris, C. C., Pegg, A., Grafstrom, R. C., & Trump, B. F. (1984). O6-alkylguanine-DNA alkyltransferase activity in normal human tissues and cells. Cancer Research, 44(7), 2855-2857.
Harris, Curtis C. ; Pegg, Anthony ; Grafstrom, Roland C. ; Trump, Benjamin F. / O6-alkylguanine-DNA alkyltransferase activity in normal human tissues and cells. In: Cancer Research. 1984 ; Vol. 44, No. 7. pp. 2855-2857.
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Harris, CC, Pegg, A, Grafstrom, RC & Trump, BF 1984, 'O6-alkylguanine-DNA alkyltransferase activity in normal human tissues and cells', Cancer Research, vol. 44, no. 7, pp. 2855-2857.

O6-alkylguanine-DNA alkyltransferase activity in normal human tissues and cells. / Harris, Curtis C.; Pegg, Anthony; Grafstrom, Roland C.; Trump, Benjamin F.

In: Cancer Research, Vol. 44, No. 7, 01.07.1984, p. 2855-2857.

Research output: Contribution to journalArticle

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