Obesity inhibits the osteogenic differentiation of human adipose-derived stem cells

Amy L. Strong, Ryan S. Hunter, Robert B. Jones, Annie C. Bowles, Maria F. Dutreil, Dina Gaupp, Daniel J. Hayes, Jeffrey M. Gimble, Benjamin Levi, Margaret A. McNulty, Bruce A. Bunnell

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Abstract

Background: Craniomaxillofacial defects secondary to trauma, tumor resection, or congenital malformations are frequent unmet challenges, due to suboptimal alloplastic options and limited autologous tissues such as bone. Significant advances have been made in the application of adipose-derived stem/stromal cells (ASCs) in the pre-clinical and clinical settings as a cell source for tissue engineering approaches. To fully realize the translational potential of ASCs, the identification of optimal donors for ASCs will ensure the successful implementation of these cells for tissue engineering approaches. In the current study, the impact of obesity on the osteogenic differentiation of ASCs was investigated. Methods: ASCs isolated from lean donors (body mass index <25; lnASCs) and obese donors (body mass index >30; obASCs) were induced with osteogenic differentiation medium as monolayers in an estrogen-depleted culture system and on three-dimensional scaffolds. Critical size calvarial defects were generated in male nude mice and treated with scaffolds implanted with lnASCs or obASCs. Results: lnASCs demonstrated enhanced osteogenic differentiation in monolayer culture system, on three-dimensional scaffolds, and for the treatment of calvarial defects, whereas obASCs were unable to induce similar levels of osteogenic differentiation in vitro and in vivo. Gene expression analysis of lnASCs and obASCs during osteogenic differentiation demonstrated higher levels of osteogenic genes in lnASCs compared to obASCs. Conclusion: Collectively, these results indicate that obesity reduces the osteogenic differentiation capacity of ASCs such that they may have a limited suitability as a cell source for tissue engineering.

Original languageEnglish (US)
Article number27
JournalJournal of Translational Medicine
Volume14
Issue number1
DOIs
StatePublished - Jan 27 2016

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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    Strong, A. L., Hunter, R. S., Jones, R. B., Bowles, A. C., Dutreil, M. F., Gaupp, D., Hayes, D. J., Gimble, J. M., Levi, B., McNulty, M. A., & Bunnell, B. A. (2016). Obesity inhibits the osteogenic differentiation of human adipose-derived stem cells. Journal of Translational Medicine, 14(1), [27]. https://doi.org/10.1186/s12967-016-0776-1