Observation and quantitation of exocytosis from the cell body of a fully developed neuron in Planorbis corneus

Guangyao Chen, Peter F. Gavin, Guoan Luo, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

We have discovered a neuronal system that releases neurotransmitter via exocytosis from the cell body. In the large dopamine cell of the pond snail Planorbis corneus, depolarization induces rhythmic release of dopamine from the cell body. When a stimulant is applied extracellularly or intracellularly in situ to the cell body, transient dopamine concentration packets that appear in a bursting pattern are observed. Dopamine release is calcium dependent and release is on the time scale expected for exocytosis (2 to 4 msec rise times). Quantitation of individual events reveals an average of 818,000 molecules per exocytotic event. As many as 89,000 individual exocytotic events have been observed following a single stimulation of one cell. Neurotransmitter exocytosis from the neuronal cell body appears to represent an alternative form of neurocommunication to synaptic transmission.

Original languageEnglish (US)
Pages (from-to)7747-7755
Number of pages9
JournalJournal of Neuroscience
Volume15
Issue number11
DOIs
StatePublished - Nov 1995

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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