Occludin localizes to centrosomes and modifies mitotic entry

E. Aaron Runkle, Jeffrey Sundstrom, Kristin B. Runkle, Xuwen Liu, David A. Antonetti

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Proper control of cell cycle progression and barrier function are essential processes to the maintenance of epithelial cell homeostasis. The contribution of tight junction proteins to barrier function is well established, whereas their contribution to cell cycle control is only beginning to be understood. Centrosomes are the principal microtubule organizing centers in eukaryotic cells and centrosome duplication and separation are linked to the cell cycle and mitotic entry. Here we demonstrate that occludin localizes with centrosomes in Madin-Darby canine kidney cells. Immunocytochemistry and biochemical fractionation studies reveal occludin localizes with centrosomes during interphase and occludin Ser-490 phosphorylation at centrosomes increases with mitotic entry. Stable expression of aspartic acid phosphomimetic (S490D) results in centrosomal localization of occludin and increases cell numbers. Furthermore, we provide evidence that occludin regulates centrosome separation and mitotic entry as the nonphosphorylatable alanine mutation (S490A) impedes centrosome separation, delays mitotic entry, and reduces proliferation. Collectively, these studies demonstrate a novel location and function for occludin in centrosome separation and mitosis.

Original languageEnglish (US)
Pages (from-to)30847-30858
Number of pages12
JournalJournal of Biological Chemistry
Volume286
Issue number35
DOIs
StatePublished - Sep 2 2011

Fingerprint

Occludin
Centrosome
Cells
Cell Cycle Checkpoints
Tight Junction Proteins
Phosphorylation
Microtubule-Organizing Center
Fractionation
Aspartic Acid
Alanine
Madin Darby Canine Kidney Cells
Interphase
Eukaryotic Cells
Mitosis
Cell Cycle
Homeostasis
Cell Count
Epithelial Cells
Immunohistochemistry
Maintenance

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Runkle, E. Aaron ; Sundstrom, Jeffrey ; Runkle, Kristin B. ; Liu, Xuwen ; Antonetti, David A. / Occludin localizes to centrosomes and modifies mitotic entry. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 35. pp. 30847-30858.
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Occludin localizes to centrosomes and modifies mitotic entry. / Runkle, E. Aaron; Sundstrom, Jeffrey; Runkle, Kristin B.; Liu, Xuwen; Antonetti, David A.

In: Journal of Biological Chemistry, Vol. 286, No. 35, 02.09.2011, p. 30847-30858.

Research output: Contribution to journalArticle

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