Occurrence and induction of spermidine-N1-acetyltransferase in Escherichia coli

Isao Matsui, Masaharu Kamei, Shuzo Otani, Seiji Morisawa, Anthony Pegg

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Spermidine acetylase activity was detected in extracts prepared from Escherichia coli and there was a marked increase in activity over the early period of growth. This increase reached a maximum 3 h after inoculation and was followed by an increase in ornithine decarboxylase activity. The acetylase was also able to use spermine as a substrate, but not putrescine. With spermidine and acetyl-CoA as substrate, the product formed was exclusively N1-acetyl-spermidine. This is the first evidence for the occurrence in bacteria of spermidine-N1-acetyltransferase, an enzyme which has previously been described in mammalian cells. These results suggest that acetylation of spermidine may be involved in the growth of Escherichia coli and in the regulation of its polyamine content.

Original languageEnglish (US)
Pages (from-to)1155-1160
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume106
Issue number4
DOIs
StatePublished - Jun 30 1982

Fingerprint

Spermidine
Escherichia coli
Acetylesterase
Acetylation
Acetyl Coenzyme A
Ornithine Decarboxylase
Putrescine
Spermine
Polyamines
Substrates
Growth
Bacteria
Cells
Enzymes
diamine N-acetyltransferase

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Matsui, Isao ; Kamei, Masaharu ; Otani, Shuzo ; Morisawa, Seiji ; Pegg, Anthony. / Occurrence and induction of spermidine-N1-acetyltransferase in Escherichia coli. In: Biochemical and Biophysical Research Communications. 1982 ; Vol. 106, No. 4. pp. 1155-1160.
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Occurrence and induction of spermidine-N1-acetyltransferase in Escherichia coli. / Matsui, Isao; Kamei, Masaharu; Otani, Shuzo; Morisawa, Seiji; Pegg, Anthony.

In: Biochemical and Biophysical Research Communications, Vol. 106, No. 4, 30.06.1982, p. 1155-1160.

Research output: Contribution to journalArticle

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