Offspring production of ovarian organoids derived from spermatogonial stem cells by defined factors with chromatin reorganization

Huacheng Luo, Xiaoyong Li, Geng G. Tian, Dali Li, Changliang Hou, Xinbao Ding, Lin Hou, Qifeng Lyu, Yunze Yang, Austin J. Cooney, Wenhai Xie, Ji Xiong, Hu Wang, Xiaodong Zhao, Ji Wu

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: Fate determination of germline stem cells remains poorly understood at the chromatin structure level. Objectives: Our research hopes to develop successful offspring production of ovarian organoids derived from spermatogonial stem cells (SSCs) by defined factors. Methods: The offspring production from oocytes transdifferentiated from mouse SSCs with tracking of transplanted SSCs in vivo, single cell whole exome sequencing, and in 3D cell culture reconstitution of the process of oogenesis derived from SSCs. The defined factors were screened with ovarian organoids. We uncovered extensive chromatin reorganization during SSC conversion into induced germline stem cells (iGSCs) using high throughput chromosome conformation. Results: We demonstrate successful production of offspring from oocytes transdifferentiated from mouse spermatogonial stem cells (SSCs). Furthermore, we demonstrate direct induction of germline stem cells (iGSCs) differentiated into functional oocytes by transduction of H19, Stella, and Zfp57 and inactivation of Plzf in SSCs after screening with ovarian organoids. We uncovered extensive chromatin reorganization during SSC conversion into iGSCs, which was highly similar to female germline stem cells. We observed that although topologically associating domains were stable during SSC conversion, chromatin interactions changed in a striking manner, altering 35% of inactive and active chromosomal compartments throughout the genome. Conclusion: We demonstrate successful offspring production of ovarian organoids derived from SSCs by defined factors with chromatin reorganization. These findings have important implications in various areas including mammalian gametogenesis, genetic and epigenetic reprogramming, biotechnology, and medicine.

Original languageEnglish (US)
JournalJournal of Advanced Research
DOIs
StateAccepted/In press - 2021

All Science Journal Classification (ASJC) codes

  • General

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