Omega-3 Fatty Acids Responsive Proteins and Reduction in Breast Density in Obese Postmenopausal Women

Yuan Wan Sun, Haifang Xu, Gabrielle Benitez, Kun Ming Chen, Anne Stanley, Bruce Stanley, Junjia Zhu, Henry Thompson, Andrea Manni, Karam El-Bayoumy

Research output: Contribution to journalArticlepeer-review

Abstract

We reported that breast density (BD) was inversely correlated with the plasma level of DHA in postmenopausal obese, but not in nonobese, women given Lovaza (n-3FA). To identify protein biomarkers for the possible differential effect of n-3FA on BD between obese and nonobese women, an iTRAQ method was performed to analyze plasma from obese and lean women at each time point (baseline, 12 and 24-months, n = 10 per group); 173 proteins with >95% confidence (Unuses Score >1.3 and local false discovery rate estimation <5%) were identified. Comparative analysis between various groups identified several differentially expressed proteins (hemopexin precursor, vitamin D binding protein isoform 1 precursor [VDBP], fibronectin isoform 10 precursor [FN], and α-2 macroglobulin precursor [A2M]). Western blot analysis was performed to verify the differential expression of proteins in the iTRAQ study, and those found to be altered in a tumor protective fashion by an n-3FA rich diet in our previous preclinical study; gelsolin, VDBP, and FN were altered by n-3FA in a manner consistent with reduction in inflammation in obese women. To test the impact of our findings on breast cancer risk reduction by n-3FA, a posthoc analysis revealed that n-3FA administration reduced BD selectively in obese postmenopausal women.

Original languageEnglish (US)
Pages (from-to)3461-3469
Number of pages9
JournalJournal of Proteome Research
Volume18
Issue number9
DOIs
StatePublished - Sep 6 2019

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

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