Abstract

We reported that breast density (BD) was inversely correlated with the plasma level of DHA in postmenopausal obese, but not in nonobese, women given Lovaza (n-3FA). To identify protein biomarkers for the possible differential effect of n-3FA on BD between obese and nonobese women, an iTRAQ method was performed to analyze plasma from obese and lean women at each time point (baseline, 12 and 24-months, n = 10 per group); 173 proteins with >95% confidence (Unuses Score >1.3 and local false discovery rate estimation <5%) were identified. Comparative analysis between various groups identified several differentially expressed proteins (hemopexin precursor, vitamin D binding protein isoform 1 precursor [VDBP], fibronectin isoform 10 precursor [FN], and α-2 macroglobulin precursor [A2M]). Western blot analysis was performed to verify the differential expression of proteins in the iTRAQ study, and those found to be altered in a tumor protective fashion by an n-3FA rich diet in our previous preclinical study; gelsolin, VDBP, and FN were altered by n-3FA in a manner consistent with reduction in inflammation in obese women. To test the impact of our findings on breast cancer risk reduction by n-3FA, a posthoc analysis revealed that n-3FA administration reduced BD selectively in obese postmenopausal women.

Original languageEnglish (US)
Pages (from-to)3461-3469
Number of pages9
JournalJournal of Proteome Research
Volume18
Issue number9
DOIs
StatePublished - Sep 6 2019

Fingerprint

Omega-3 Fatty Acids
Vitamin D-Binding Protein
Protein Isoforms
Proteins
Hemopexin
Gelsolin
Plasmas
Macroglobulins
Biomarkers
Nutrition
Fibronectins
Protein Precursors
Tumors
Risk Reduction Behavior
Western Blotting
Breast Density
Breast Neoplasms
Diet
Inflammation
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

Cite this

@article{0d55d60494054b53b5872319f6874cdf,
title = "Omega-3 Fatty Acids Responsive Proteins and Reduction in Breast Density in Obese Postmenopausal Women",
abstract = "We reported that breast density (BD) was inversely correlated with the plasma level of DHA in postmenopausal obese, but not in nonobese, women given Lovaza (n-3FA). To identify protein biomarkers for the possible differential effect of n-3FA on BD between obese and nonobese women, an iTRAQ method was performed to analyze plasma from obese and lean women at each time point (baseline, 12 and 24-months, n = 10 per group); 173 proteins with >95{\%} confidence (Unuses Score >1.3 and local false discovery rate estimation <5{\%}) were identified. Comparative analysis between various groups identified several differentially expressed proteins (hemopexin precursor, vitamin D binding protein isoform 1 precursor [VDBP], fibronectin isoform 10 precursor [FN], and α-2 macroglobulin precursor [A2M]). Western blot analysis was performed to verify the differential expression of proteins in the iTRAQ study, and those found to be altered in a tumor protective fashion by an n-3FA rich diet in our previous preclinical study; gelsolin, VDBP, and FN were altered by n-3FA in a manner consistent with reduction in inflammation in obese women. To test the impact of our findings on breast cancer risk reduction by n-3FA, a posthoc analysis revealed that n-3FA administration reduced BD selectively in obese postmenopausal women.",
author = "Yuan-Wan Sun and Haifang Xu and Gabrielle Benitez and Chen, {Kun Ming} and Anne Stanley and Bruce Stanley and Junjia Zhu and Henry Thompson and Andrea Manni and Karam El-Bayoumy",
year = "2019",
month = "9",
day = "6",
doi = "10.1021/acs.jproteome.9b00356",
language = "English (US)",
volume = "18",
pages = "3461--3469",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "9",

}

Omega-3 Fatty Acids Responsive Proteins and Reduction in Breast Density in Obese Postmenopausal Women. / Sun, Yuan-Wan; Xu, Haifang; Benitez, Gabrielle; Chen, Kun Ming; Stanley, Anne; Stanley, Bruce; Zhu, Junjia; Thompson, Henry; Manni, Andrea; El-Bayoumy, Karam.

In: Journal of Proteome Research, Vol. 18, No. 9, 06.09.2019, p. 3461-3469.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Omega-3 Fatty Acids Responsive Proteins and Reduction in Breast Density in Obese Postmenopausal Women

AU - Sun, Yuan-Wan

AU - Xu, Haifang

AU - Benitez, Gabrielle

AU - Chen, Kun Ming

AU - Stanley, Anne

AU - Stanley, Bruce

AU - Zhu, Junjia

AU - Thompson, Henry

AU - Manni, Andrea

AU - El-Bayoumy, Karam

PY - 2019/9/6

Y1 - 2019/9/6

N2 - We reported that breast density (BD) was inversely correlated with the plasma level of DHA in postmenopausal obese, but not in nonobese, women given Lovaza (n-3FA). To identify protein biomarkers for the possible differential effect of n-3FA on BD between obese and nonobese women, an iTRAQ method was performed to analyze plasma from obese and lean women at each time point (baseline, 12 and 24-months, n = 10 per group); 173 proteins with >95% confidence (Unuses Score >1.3 and local false discovery rate estimation <5%) were identified. Comparative analysis between various groups identified several differentially expressed proteins (hemopexin precursor, vitamin D binding protein isoform 1 precursor [VDBP], fibronectin isoform 10 precursor [FN], and α-2 macroglobulin precursor [A2M]). Western blot analysis was performed to verify the differential expression of proteins in the iTRAQ study, and those found to be altered in a tumor protective fashion by an n-3FA rich diet in our previous preclinical study; gelsolin, VDBP, and FN were altered by n-3FA in a manner consistent with reduction in inflammation in obese women. To test the impact of our findings on breast cancer risk reduction by n-3FA, a posthoc analysis revealed that n-3FA administration reduced BD selectively in obese postmenopausal women.

AB - We reported that breast density (BD) was inversely correlated with the plasma level of DHA in postmenopausal obese, but not in nonobese, women given Lovaza (n-3FA). To identify protein biomarkers for the possible differential effect of n-3FA on BD between obese and nonobese women, an iTRAQ method was performed to analyze plasma from obese and lean women at each time point (baseline, 12 and 24-months, n = 10 per group); 173 proteins with >95% confidence (Unuses Score >1.3 and local false discovery rate estimation <5%) were identified. Comparative analysis between various groups identified several differentially expressed proteins (hemopexin precursor, vitamin D binding protein isoform 1 precursor [VDBP], fibronectin isoform 10 precursor [FN], and α-2 macroglobulin precursor [A2M]). Western blot analysis was performed to verify the differential expression of proteins in the iTRAQ study, and those found to be altered in a tumor protective fashion by an n-3FA rich diet in our previous preclinical study; gelsolin, VDBP, and FN were altered by n-3FA in a manner consistent with reduction in inflammation in obese women. To test the impact of our findings on breast cancer risk reduction by n-3FA, a posthoc analysis revealed that n-3FA administration reduced BD selectively in obese postmenopausal women.

UR - http://www.scopus.com/inward/record.url?scp=85071607330&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071607330&partnerID=8YFLogxK

U2 - 10.1021/acs.jproteome.9b00356

DO - 10.1021/acs.jproteome.9b00356

M3 - Article

C2 - 31369706

AN - SCOPUS:85071607330

VL - 18

SP - 3461

EP - 3469

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 9

ER -