On-column and post-column derivatization for capillary electrophoresis with laser-induced fluorescence for the analysis of single cells

Paula Beyer Hietpas, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

Derivatization methods for analysis of single cell components by capillary electrophoresis with laser-induced fluorescence detection are reviewed. Recent advances have lead to the use of this technique for the identification of amino acids and proteins in many different cell lines ranging in size from 8 to 140 um Pre-column, on-column, and post-column methods of derivatization are examined in order to determine which results in the least amount of sample handling and dilution leading to better detection limits for the analytes of interest.

Original languageEnglish (US)
Pages (from-to)3557-3576
Number of pages20
JournalJournal of Liquid Chromatography
Volume18
Issue number18-19
DOIs
StatePublished - Nov 1 1995

All Science Journal Classification (ASJC) codes

  • Molecular Medicine

Fingerprint Dive into the research topics of 'On-column and post-column derivatization for capillary electrophoresis with laser-induced fluorescence for the analysis of single cells'. Together they form a unique fingerprint.

Cite this