On the essentiality of lipopolysaccharide to Gram-negative bacteria

Ge Zhang, Timothy Charles Meredith, Daniel Kahne

Research output: Contribution to journalReview article

71 Citations (Scopus)

Abstract

Lipopolysaccharide is a highly acylated saccharolipid located on the outer leaflet of the outer membrane of Gram-negative bacteria. Lipopolysaccharide is critical to maintaining the barrier function preventing the passive diffusion of hydrophobic solutes such as antibiotics and detergents into the cell. Lipopolysaccharide has been considered an essential component for outer membrane biogenesis and cell viability based on pioneering studies in the model Gram-negative organisms Escherichia coli and Salmonella. With the isolation of lipopolysaccharide-null mutants in Neisseria meningitidis, Moraxella catarrhalis, and most recently in Acinetobacter baumannii, it has become increasingly apparent that lipopolysaccharide is not an essential outer membrane building block in all organisms. We suggest the accumulation of toxic intermediates, misassembly of essential outer membrane porins, and outer membrane stress response pathways that are activated by mislocalized lipopolysaccharide may collectively contribute to the observed strain-dependent essentiality of lipopolysaccharide.

Original languageEnglish (US)
Pages (from-to)779-785
Number of pages7
JournalCurrent Opinion in Microbiology
Volume16
Issue number6
DOIs
StatePublished - Dec 1 2013

Fingerprint

Gram-Negative Bacteria
Lipopolysaccharides
Membranes
Moraxella (Branhamella) catarrhalis
Acinetobacter baumannii
Porins
Neisseria meningitidis
Poisons
Salmonella
Detergents
Cell Survival
Escherichia coli
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

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On the essentiality of lipopolysaccharide to Gram-negative bacteria. / Zhang, Ge; Meredith, Timothy Charles; Kahne, Daniel.

In: Current Opinion in Microbiology, Vol. 16, No. 6, 01.12.2013, p. 779-785.

Research output: Contribution to journalReview article

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