On the existence of polyadenylated histone mRNA in Xenopus laevis oocytes

Robert Levenson, Kenneth B. Marcu

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

In a variety of systems, histone mRNA has been shown to lack poly(A) (Adesnik and Darnell, 1972;Grunstein et al., 1973). We have found, however, that in Xenopus laevis oocytes, poly(A)-containing mRNA codes for histones, in a wheat germ cell-free system, based on the following criteria: first, co-migration with authentic X. laevis oocyte histones on polyacrylamide gels; second, no detectable incorporation of tryptophan; third, differential incorporation of lysine and methionine into histone fraction H2A; fourth, resistance of histone fraction H2A to cleavage with cyanogen bromide; and fifth, correspondence of tryptic peptide maps of partially purified cell-free products with authentic X. laevis oocyte histone. RNA which directs the synthesis of histones in the cell-free system is retained on oligo(dT)-cellulose, even after denaturation in 80% DMSO at 70°C, thereby demonstrating the covalent attachment of polyadenylic acid sequences to the mRNA. Poly(A)- RNA (7S-14S fraction) was also found to code for histones using the same criteria. We discuss the significance of the finding that X. laevis oocytes contain two classes of histone mRNA as well as the potential developmental implications of this observation.

Original languageEnglish (US)
Pages (from-to)311-322
Number of pages12
JournalCell
Volume9
Issue number2
DOIs
StatePublished - Jan 1 1976

Fingerprint

Xenopus laevis
Histones
Oocytes
Messenger RNA
Histone Code
Poly A
Cell-Free System
Cyanogen Bromide
Dimethyl Sulfoxide
Germ Cells
Tryptophan
Methionine
Denaturation
Lysine
Triticum
RNA
Peptides
Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Levenson, Robert ; Marcu, Kenneth B. / On the existence of polyadenylated histone mRNA in Xenopus laevis oocytes. In: Cell. 1976 ; Vol. 9, No. 2. pp. 311-322.
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On the existence of polyadenylated histone mRNA in Xenopus laevis oocytes. / Levenson, Robert; Marcu, Kenneth B.

In: Cell, Vol. 9, No. 2, 01.01.1976, p. 311-322.

Research output: Contribution to journalArticle

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